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CRALBP Antibody (B2)

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Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.
Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.
Immunocytochemistry/ Immunofluorescence: CRALBP Antibody (B2) [NB100-74392] - Immunostaining of CRALBP in frozen mouse retina sections.
Simple Western: CRALBP Antibody (B2) [NB100-74392] - iPSC-RPE from all donors express RPE marker proteins. Unlike iPSCs, all iPSC-RPE lines showed robust expression of CRALBP. Image collected and cropped by CiteAb from ...read more
Phd2 deletion leads to activation of the Akt–mTOR pathway. a RPPA analysis of tumors with homozygous deletion of Phd2. Tumor tissues from Tyr::CreER; BRafV600E; Phd2−/− or Tyr::CreER; BRafV600E mice were processed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP
Clone
B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CRALBP Antibody (B2) Summary

Immunogen
Human recombinant CRALBP.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RLBP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 0.02 ug/ml
Application Notes
ELISA, IP, WB reported in the literature (PMID: 1715867) ICC reported in the literature (PMID:11909957)
Control
CRALBP Overexpression Lysate
Publications
Read Publications using
NB100-74392 in the following applications:

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:35327471).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CRALBP Antibody (B2)

  • Cellular retinaldehyde-binding protein
  • cellular retinaldehyde-binding protein-1
  • CRALBPretinaldehyde-binding protein 1
  • MGC3663
  • retinaldehyde binding protein 1

Background

The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CRALBP Antibody
(B2)
NB100-74392
CRALBP Antibody (B2)
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP

Publications for CRALBP Antibody (NB100-74392)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, Simple Western, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(3)
IHC-P
(2)
Simple Western
(1)
WB
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Rat
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-74392 Applications Species
Farkas MH, Skelton LA, Ramachandra-Rao S et al. Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients Molecular Vision 2022-01-01 [PMID: 36540063] (IHC-P, Human)

Details:
1:100 dilution IHC-P 		IHC-P Human
Lytvynchuk L, Ebbert A, Studenovska H et al. Subretinal Implantation of Human Primary RPE Cells Cultured on Nanofibrous Membranes in Minipigs Biomedicines 2022-03-14 [PMID: 35327471] (IF/IHC, Porcine) IF/IHC Porcine
Navneet, S;Zhao, J;Wang, J;Mysona, B;Barwick, S;Ammal Kaidery, N;Saul, A;Kaddour-Djebbar, I;Bollag, WB;Thomas, B;Bollinger, KE;Smith, SB; Hyperhomocysteinemia-induced death of retinal ganglion cells: The role of Muller glial cells and NRF2 Redox Biol 2019-04-11 [PMID: 31026769] (IF/IHC, Mouse) IF/IHC Mouse
Navneet S, Cui X, Zhao J et al. Excess homocysteine upregulates the NRF2-antioxidant pathway in retinal Muller glial cells. Exp Eye Res. [PMID: 29608906] (ICC/IF, Mouse) ICC/IF Mouse
Golestaneh Nady, Chu Yi, Xiao Yang-Yu et al. Dysfunctional autophagy in RPE, a contributing factor in age-related macular degeneration. Cell Death & Disease 2017-01-05 [PMID: 28055007] (ICC/IF, Human) ICC/IF Human
Biesecker Kr, Srienc Ai, Shimoda Am et al. Glial Cell Calcium Signaling Mediates Capillary Regulation of Blood Flow in the Retina. J. Neurosci. 2016-09-07 [PMID: 27605617] (IHC-P) IHC-P
Heller JP, Kwok JC, Vecino E et al. A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases. Front Cell Neurosci 2015-01-01 [PMID: 26635529] (Rat) Rat
Unterlauft JD, Eichler W, Kuhne K et al. Pigment Epithelium-Derived Factor Released by Muller Glial Cells Exerts Neuroprotective Effects on Retinal Ganglion Cells Neurochem Res 2012-03-13 [PMID: 22410737] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Arnold TD, Ferrero GM, Qiu H et al. Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin [alpha]V[beta]8-Mediated Activation of Transforming Growth Factor-[beta]. J Neurosci 2532(4):1197-12. 2012-01-01 [PMID: 22279205]
Lu B, Malcuit C, Wang S et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration. Stem Cells 2009-09-01 [PMID: 19521979] (WB, Human) WB Human
Show All 14 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRALBP Antibody (NB100-74392) (0)

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Control Lysate(s)

CRALBP Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RLBP1
Entrez
Uniprot