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COP9 Antibody

Images

 
Western Blot: COP9 Antibody [NBP1-85435] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: COP9 Antibody [NBP1-85435] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: COP9 Antibody [NBP1-85435] - Staining of human cerebellum shows strong nuclear positivity in purkinje cells.
Western Blot: COP9 Antibody [NBP1-85435] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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COP9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COPS3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
COP9 Protein (NBP1-85435PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for COP9 Antibody

  • COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
  • COP9 signalosome complex subunit 3
  • CSN3COP9 complex subunit 3
  • JAB1-containing signalosome subunit 3
  • SGN3COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 3
  • Signalosome subunit 3

Background

COP9 is encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COP9 Antibody (NBP1-85435) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COP9 Antibody (NBP1-85435) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COPS3