COP9 Antibodies
We offer COP9 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunodiffusion, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each COP9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our COP9 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the COP9 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our COP9 polyclonal antibodies and browse our COP9 monoclonal antibody catalog.
Alternate Names for COP9 Antibodies
anti-COP9 antibody, anti-COPS3 antibody, anti-COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) antibody, anti-COP9 signalosome complex subunit 3 antibody, anti-CSN3COP9 complex subunit 3 antibody, anti-JAB1-containing signalosome subunit 3 antibody, anti-SGN3COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 3 antibody, anti-Signalosome subunit 3 antibody
13 Results for "cop9" in Primary Antibodies
![Western Blot: COP9 Antibody [NBP1-85435] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NBP1-85435-img0007.jpg "Western Blot: COP9 Antibody [NBP1-85435] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: COP9 Antibody [NBP1-85435] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85435-img0008.jpg)
![Immunohistochemistry-Paraffin: COP9 Antibody [NBP1-85435] - Staining of human cerebellum shows strong nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunohistochemistry-Paraffin-NBP1-85435-img0002.jpg)
![Western Blot: COP9 Antibody [NBP1-85435] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NBP1-85435-img0005.jpg)
![Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-26220241422527.jpg "Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-26220241535576.jpg)
![Western Blot: COP9 Antibody (3F9) [NBP3-26645] - Positive Western Blot detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: COP9 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 46 kDa Observed band size: 40-55 kDa](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-282202411472142.jpg)
![Immunocytochemistry/Immunofluorescence: COP9 Antibody (3F9) [NBP3-26645] - Staining of A549 cell with COP9 Antibody (3F9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 535-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-2822024132364.jpg)
![Flow Cytometry: COP9 Antibody (3F9) [NBP3-26645] - Overlay Peak curve showing MCF7 cells stained with COP9 Antibody (3F9) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-282202414312713.jpg)
![Western Blot: COP9 Antibody (2D10) [H00008533-M02] - COPS3 monoclonal antibody (M02), clone 2D10. Analysis of COPS3 expression in PC-12.](http://images.novusbio.com/fullsize/COP9-Antibody-2D10-Western-Blot-H00008533-M02-img0009.jpg "Western Blot: COP9 Antibody (2D10) [H00008533-M02] - COPS3 monoclonal antibody (M02), clone 2D10. Analysis of COPS3 expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: COP9 Antibody (2D10) [H00008533-M02] - Analysis of monoclonal antibody to COPS3 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/COP9-Antibody-2D10-Immunocytochemistry-Immunofluorescence-H00008533-M02-img0006.jpg)
![Western Blot: COP9 Antibody (2D10) [H00008533-M02] - COPS3 monoclonal antibody (M02), clone 2D10 Analysis of COPS3 expression in HeLa.](http://images.novusbio.com/fullsize/COP9-Antibody-2D10-Western-Blot-H00008533-M02-img0008.jpg)
![Western Blot: COP9 Antibody (2D10) [H00008533-M02] - Analysis of COPS3 expression in transfected 293T cell line by COPS3 monoclonal antibody (M02), clone 2D10.Lane 1: COPS3 transfected lysate(47.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COP9-Antibody-2D10-Western-Blot-H00008533-M02-img0010.jpg)
![Sandwich ELISA: COP9 Antibody (2D10) [H00008533-M02] - Detection limit for recombinant GST tagged COPS3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/COP9-Antibody-2D10-Sandwich-ELISA-H00008533-M02-img0007.jpg)
![Western Blot: COP9 Antibody (7V1L8) [NBP3-15791] - Western blot analysis of extracts of various cell lines, using COP9 Rabbit mAb (NBP3-15791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/COP9-Antibody-7V1L8-Western-Blot-NBP3-15791-img0004.jpg "Western Blot: COP9 Antibody (7V1L8) [NBP3-15791] - Western blot analysis of extracts of various cell lines, using COP9 Rabbit mAb (NBP3-15791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: COP9 Antibody (7V1L8) [NBP3-15791] - Immunohistochemistry of paraffin-embedded mouse heart using COP9 Rabbit mAb (NBP3-15791) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COP9-Antibody-7V1L8-Immunohistochemistry-Paraffin-NBP3-15791-img0003.jpg)
![Immunohistochemistry-Paraffin: COP9 Antibody (7V1L8) [NBP3-15791] - Immunohistochemistry of paraffin-embedded rat heart using COP9 Rabbit mAb (NBP3-15791) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COP9-Antibody-7V1L8-Immunohistochemistry-Paraffin-NBP3-15791-img0001.jpg)
![Immunohistochemistry-Paraffin: COP9 Antibody (7V1L8) [NBP3-15791] - Immunohistochemistry of paraffin-embedded human colon using COP9 Rabbit mAb (NBP3-15791) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COP9-Antibody-7V1L8-Immunohistochemistry-Paraffin-NBP3-15791-img0002.jpg)
![Western Blot: COP9 Antibody [NBP2-15956] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NBP2-15956-img0001.jpg "Western Blot: COP9 Antibody [NBP2-15956] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: COP9 Antibody [NBP2-15956] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COP9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15956-img0003.jpg)
![Immunohistochemistry-Paraffin: COP9 Antibody [NBP2-15956] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunohistochemistry-Paraffin-NBP2-15956-img0002.jpg)
![Western Blot: COP9 Antibody [NBP3-38362] - Western blot analysis of various lysates using COP9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38362_rabbit-cop9-pab-101220241136022.jpg "Western Blot: COP9 Antibody [NBP3-38362] - Western blot analysis of various lysates using COP9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: COP9 Antibody [NBP3-38362] - Immunofluorescence analysis of MCF7 cells using COP9 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38362_rabbit-cop9-pab-1012202411353090.jpg)
![Western Blot: COP9 Antibody (SR1823) [NBP3-22048] - Western blot analysis using NBP3-22048 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22048_rabbit-cop9-mab-sr1823-57202316494268.jpg "Western Blot: COP9 Antibody (SR1823) [NBP3-22048] - Western blot analysis using NBP3-22048 in HeLa cell lysate.")
![Western Blot: COP9 Antibody [NBP2-55300] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NBP2-55300-img0002.jpg "Western Blot: COP9 Antibody [NBP2-55300] - Western blot analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: COP9 Antibody [NBP2-55300] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55300-img0001.jpg)
![Western Blot: COP9 Antibody [NB100-365] - Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody NB100-365 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NB100-365-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: COP9 Antibody [NB100-365] - Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody NB100-365 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: COP9 Antibody [NB100-365] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN3 transfected (T) HEK293T cells. Antibody used at the indicated concentration.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NB100-365-img0002.jpg)
![Western Blot: COP9 Antibody (OTI3E1) [NBP2-46332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COPS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiCOPS3.(1:2000](http://images.novusbio.com/fullsize/COP9-Antibody-OTI3E1-Western-Blot-NBP2-46332-img0002.jpg "Western Blot: COP9 Antibody (OTI3E1) [NBP2-46332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COPS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiCOPS3.(1:2000")