![Immunocytochemistry/Immunofluorescence: Complement Component C3aR Antibody (3G7) [NBP2-81062] - Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81062, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C3aR-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP2-81062-img0001.jpg "Immunocytochemistry/Immunofluorescence: Complement Component C3aR Antibody (3G7) [NBP2-81062] - Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81062, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | 3G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The 2e loop of the Complement Component C3aR. |
| Specificity | Binds specifically to the 2e loop of the human C3a receptor, residues 185-193. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | C3AR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This reformatted mouse antibody was made using the variable domain sequences of the original mouse scFv format for improved compatibility with existing reagents, assays and techniques. This scFv antibody binds to the 2e loop of the human C3a receptor, a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Complement Component C3aR Antibody (NBP2-81062)Find related products by research area.
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| Gene Symbol | C3AR1 |
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