Mouse Complement C3a ELISA Kit (Colorimetric)

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1). C3 is cleaved by C3 convertase into two fragments, the smaller C3a and larger C3b (1,2). Human C3a is 77 amino acids (aa) in length and has a theoretical molecular weight of ~9 kDa (2). Structurally, complement C3a consists of four anti-parallel helical structures with three disulfide bridges (2). The C3a fragment binds the G-protein coupled receptor (GPCR) C3a (C3aR) (2,3). Functionally, C3a plays an important role in regulating both adaptive immunity and innate immunity (1-3).

The C3a/C3aR pathway is often referred to as a "double-edged sword" because it has both anti-inflammatory and pro-inflammatory functions (2,3). For instance, its anti-inflammatory role includes pathogen elimination and reduced interferon-gamma (IFN-gamma) expression and cytotoxicity in natural killer cells (2). By contrast, complement C3a is an anaphylatoxin and plays a role in inflammation by prompting histamine release from mast cells and increasing permeability of the vascular system (2-4). The C3a/C3aR pathway has also been indicated in a number of diseases including Alzheimer's disease, asthma, and kidney disease (3). Patients with various kidney diseases tend to show higher levels of complement C3a in the plasma and urine (3).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Gao, S., Cui, Z., & Zhao, M. H. (2020). The Complement C3a and C3a Receptor Pathway in Kidney Diseases. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01875

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Haddad, A., & Wilson, A. M. (2020). Biochemistry, Complement. In StatPearls. StatPearls Publishing.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.

Showing Publications 1 - 6 of 6.

Publications using NBP2-70037	Applications	Species
Krishnan R, Stapledon CJM, Mostafavi H et al. Anti-inflammatory actions of Pentosan polysulfate sodium in a mouse model of influenza virus A/PR8/34-induced pulmonary inflammation Frontiers in immunology 2023-02-09 [PMID: 36845136] (ELISA, Mouse)	ELISA	Mouse
Li C, Li Y, Zhang H et al. Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-kappa B Signaling Pathway Phytomedicine 2022-11-01 [PMID: 36610119] (ELISA, Mouse) Details: 1:500 dilution ICC/IF	ELISA	Mouse
Guo Z, Chen J, Zeng Y et al. Complement Inhibition Alleviates Cholestatic Liver Injury Through Mediating Macrophage Infiltration and Function in Mice Frontiers in immunology 2022-01-07 [PMID: 35069557] (ELISA)	ELISA
Nagano F, Mizuno T, Imai M Et al. Expression of a Crry/p65 is reduced in acute lung injury induced by extracellular histones FEBS open bio 2021-10-28 [PMID: 34709768] (ELISA)	ELISA
Gray L, Raczy M, Briquez P et al. Generation of potent cellular and humoral immunity against SARS-CoV-2 antigens via conjugation to a polymeric glyco-adjuvant Biomaterials 2021-09-01 [PMID: 34634664] (ELISA)	ELISA
Han R, Hu S, Qin W, et al. C3a and suPAR drive versican V1 expression in tubular cells of focal segmental glomerulosclerosis JCI Insight 2020-04-04 [PMID: 30944246] (ELISA, ELISA, Mouse)	ELISA, ELISA	Mouse

Array NBP2-70037

• Complement C3a Antibodies
• Complement C3a ELISA Kits