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Collagen VII Antibody (LH7.2) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Bv, Eq, Gp, Gt, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
LH7.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

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Collagen VII Antibody (LH7.2) [Alexa Fluor® 488] Summary

Immunogen
Recombinant full-length human Collagen VII protein (Uniprot: Q02388)
Localization
Secreted. Extracellular matrix. Basement Membrane.
Specificity
Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL7A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Collagen VII Antibody (LH7.2) [Alexa Fluor® 488]

  • Collagen 7
  • collagen alpha-1(VII) chain
  • collagen VII, alpha-1 polypeptide
  • collagen, type VII, alpha 1
  • Collagen-7
  • EBD1
  • EBDCT
  • EBR1
  • epidermolysis bullosa, dystrophic, dominant and recessive
  • LC collagen
  • long chain collagen
  • Long-chain collagen

Background

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL7A1