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Collagen VI alpha 1 Antibody

Images

 
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human placenta shows moderate positivity in extracellular matrix.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Analysis in human placenta and pancreas tissues. Corresponding COL6A1 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human testis shows weak positivity in extracellular matrix in peritubular myoid cells.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human skin shows moderate positivity in extracellular matrix.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Collagen VI alpha 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTIVDMIKNNVEQVCCSFECQPAR
Predicted Species
Mouse (95%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL6A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Collagen VI alpha 1 Recombinant Protein Antigen (NBP1-91193PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Collagen VI alpha 1 Antibody

  • Collagen 6
  • collagen alpha-1(VI) chain
  • collagen VI, alpha-1 polypeptide
  • collagen, type VI, alpha 1
  • Collagen-6
  • human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
  • OPLL

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL6A1