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Collagen VI alpha 1 Antibody (7J1P4)

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Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP ...read more
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded human liver using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x ...read more
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded rat lung using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
7J1P4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Collagen VI alpha 1 Antibody (7J1P4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 37-235 of human Collagen VI alpha 1 (P12109). DLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTI
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
COL6A1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Collagen VI alpha 1 Antibody (7J1P4)

  • Collagen 6
  • collagen alpha-1(VI) chain
  • collagen VI, alpha-1 polypeptide
  • collagen, type VI, alpha 1
  • Collagen-6
  • human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
  • OPLL

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL6A1