Collagen IV alpha 1 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

COL4A1 (collagen 4-alpha 1) is a calculated 185 kDa (ref. 1) member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes, and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length (SwissProt #:P02462). It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C‑terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958‑1669. Over aa 1441-1669, human COL4A1 shares 97% aa sequence identity to mouse COL4A1. AF6308 detects a 95 kDa band, endogenous COL4A1 by Westen blot in human placenta tissue sample, which is consistent with the current literature (ref. 1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Capture, Western Blot.

Showing Publications 1 - 2 of 2.

Publications using AF6308	Applications	Species
WR Swindell, M Randhawa, G Quijas, K Bojanowski, RK Chaudhuri Tetrahexyldecyl Ascorbate (THDC) Degrades Rapidly under Oxidative Stress but Can Be Stabilized by Acetyl Zingerone to Enhance Collagen Production and Antioxidant Effects International Journal of Molecular Sciences, 2021-08-15;22(16):. 2021-08-15 [PMID: 34445461] (ELISA Capture, Human)	ELISA Capture	Human
KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684. 2018-04-10 [PMID: 29633884] (Western Blot, Human)	Western Blot	Human

Array AF6308

• Collagen IV alpha 1 Antibodies
• Collagen IV alpha 1 ELISA Kits
• Collagen IV alpha 1 Proteins and Enzyme