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Collagen II Antibody

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Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3615
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Catalog# & Formulation Size Price
Carrier Free
AF3615-SP
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Collagen II Antibody Summary

Specificity
Detects mouse Collagen II in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
COL2A1
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3615 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen II Antibody

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen II Antibody (AF3615)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


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ICC
(1)
IHC
(1)
IHC-P
(2)
Western Blot
(2)
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Mouse
(6)
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Showing Publications 1 - 10 of 10.
Publications using AF3615 Applications Species
J Li, J Xue, Y Jing, M Wang, R Shu, H Xu, C Xue, J Feng, P Wang, D Bai SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone Biomed Res Int, 2019-11-11;2019(0):7623562. 2019-11-11 [PMID: 31828128] (IHC-P, Mouse) IHC-P Mouse
Gesine M. Scharf, Katja Kilian, Julio Cordero, Yong Wang, Andrea Grund, Melanie Hofmann et al. Inactivation of Sox9 in fibroblasts reduces cardiac fibrosis and inflammation JCI Insight 8/8/2019 [PMID: 31310588]
So EY, Sun C, Wu KQ et al. Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development J. Cell. Physiol. 7/9/2019 [PMID: 31287165]
Y Chen, H Xu, G Lin Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 2017-12-19;3(0):17046. 2017-12-19 [PMID: 29263795] (IHC, Mouse) IHC Mouse
Peng Qu, Lizhen Wang, Yongfen Min, Lois McKennett, Jonathan R. Keller, P. Charles Lin Vav1 Regulates Mesenchymal Stem Cell Differentiation Decision Between Adipocyte and Chondrocyte via Sirt1 Stem Cells 7/1/2016 [PMID: 26990002]
Alexei Y. Savinov, Maryam Salehi, Manisha C. Yadav, Ilian Radichev, José Luis Millán, Olga V. Savinova Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase (TNAP) in Vascular Endothelium Results in Generalized Arterial Calcification Journal of the American Heart Association 12/16/2015 [PMID: 26675253]
Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts. Stem Cells Int, 2014-09-10;2014(0):468927. 2014-09-10 [PMID: 25295064] (IHC-P, Mouse) IHC-P Mouse
Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease. PLoS ONE, 2012-06-07;7(6):e38615. 2012-06-07 [PMID: 22685592] (ICC, Mouse) ICC Mouse
Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription. PLoS ONE, 2012-04-12;7(4):e34703. 2012-04-12 [PMID: 22511961] (Western Blot, Mouse) Western Blot Mouse
Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism. J. Biol. Chem., 2010-12-10;286(7):5803-12. 2010-12-10 [PMID: 21148564] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol COL2A1