ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Samples were spiked with high concentrations of Rat Coagulation Factor X and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Standard Curve Reference
Product Details
Summary
Reactivity
RtSpecies Glossary
Applications
ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Rat Coagulation Factor X ELISA Kit (Colorimetric) Summary
Specificity
This kit recognizes Rat Coagulation Factor X in samples. No Significant cross-reactivity or interference between Rat Coagulation Factor X and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.4
Intra-Assay
CV% < 5.19
Spike Recovery
90-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
F10
Applications/Dilutions
Dilutions
ELISA
Publications
Read Publication using NBP2-82526 in the following applications:
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent
Alternate Names for Rat Coagulation Factor X ELISA Kit (Colorimetric)
Cf10
Coagulation Factor X
EC 3.4.21
EC 3.4.21.6
F10
factor Xa
FX
FXa
Prothrombinase
Stuart Factor
Stuart-Prower factor
Background
The Factor X gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoesmultiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of thetripeptide RKR. Two chai
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
![ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Samples were spiked with high concentrations of Rat Coagulation Factor X and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82526_rat-coagulation-factor-x-elisa-kit-colorimetric-132202416143014.jpg "ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Samples were spiked with high concentrations of Rat Coagulation Factor X and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82526_rat-coagulation-factor-x-elisa-kit-colorimetric-43202495034.jpg "ELISA: Rat Coagulation Factor X ELISA Kit (Colorimetric) [NBP2-82526] - Standard Curve Reference")
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