![Western Blot: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection against Immunogen (48.95 KDa) .](http://images.novusbio.com/fullsize/Claudin-19-Antibody-2F2-Western-Blot-H00149461-M02-img0004.jpg "Western Blot: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection against Immunogen (48.95 KDa) .")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 2F2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | CLDN19 (AAH30524, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYV |
| Specificity | CLDN19 - claudin 19 (2F2) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CLDN19 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Immunocytochemistry/Immunofluorescence was reported in scientific literature. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Claudin-19 Antibody (H00149461-M02)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins and the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. Immunostaining of the iPSC-RPE cells revealed surface expression of the claudin19. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Claudin-19-Antibody-2F2-Immunocytochemistry-Immunofluorescence-H00149461-M02-img0005.jpg "Immunocytochemistry/Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins and the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. Immunostaining of the iPSC-RPE cells revealed surface expression of the claudin19. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license.")
![ELISA: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection limit for recombinant GST tagged CLDN19 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-19-Antibody-2F2-ELISA-H00149461-M02-img0003.jpg "ELISA: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection limit for recombinant GST tagged CLDN19 is 0.1 ng/ml as a capture antibody.")
![Immunocytochemistry/ Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins & the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. a Phalloidin labeling demonstrates the cortical arrangement of actin filaments in iPSC-RPE. b–d Immunostaining of the iPSC-RPE cells revealed surface expression of the tight junction proteins ZO-1 (b), occludin (c), & claudin19 (d). e To assess the barrier function of the iPSC-RPE cells, the resistance across monolayers of cells cultured on laminin-coated Transwell inserts was measured at 2-week intervals, following initiation of the cultures. The net TER was determined by subtracting the resistance across a laminin-coated Transwell insert lacking cells, & multiplying by the surface area of the insert (0.33 cm2). The net TER of the iPSC-RPE cells steadily increased between weeks 2 & 8, until it reached a maximal value of just above 200 Ω.cm2. Resistance recordings were made from four individual cultures. e Error bars represent the mean ± the SEM. Scale bars: a–d = 20 μm. ZO-1 zonula occludens-1, TER transepithelial resistance Image collected & cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00149461-m02_mouse-monoclonal-claudin-19-antibody-2f2-310202415482262.jpg "Immunocytochemistry/ Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins & the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. a Phalloidin labeling demonstrates the cortical arrangement of actin filaments in iPSC-RPE. b–d Immunostaining of the iPSC-RPE cells revealed surface expression of the tight junction proteins ZO-1 (b), occludin (c), & claudin19 (d). e To assess the barrier function of the iPSC-RPE cells, the resistance across monolayers of cells cultured on laminin-coated Transwell inserts was measured at 2-week intervals, following initiation of the cultures. The net TER was determined by subtracting the resistance across a laminin-coated Transwell insert lacking cells, & multiplying by the surface area of the insert (0.33 cm2). The net TER of the iPSC-RPE cells steadily increased between weeks 2 & 8, until it reached a maximal value of just above 200 Ω.cm2. Resistance recordings were made from four individual cultures. e Error bars represent the mean ± the SEM. Scale bars: a–d = 20 μm. ZO-1 zonula occludens-1, TER transepithelial resistance Image collected & cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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