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LRSAM1 Antibody

Images

 
Western Blot: LRSAM1 Antibody [NBP1-82016] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-329
Immunocytochemistry/ Immunofluorescence: LRSAM1 Antibody [NBP1-82016] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human Fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LRSAM1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: REILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRSAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LRSAM1 Protein (NBP1-82016PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LRSAM1 Antibody

  • EC 6.3.2.-
  • FLJ31641
  • hTAL
  • leucine rich repeat and sterile alpha motif containing 1
  • Leucine-rich repeat and sterile alpha motif-containing protein 1
  • RIFLE
  • TALE3 ubiquitin-protein ligase LRSAM1
  • Tsg101-associated ligase

Background

LRSAM1 is a multifunctional RING finger protein that selectively regulates cell adhesion molecules, has ubiquitin ligase activity, and plays a role in receptor endocytosis and viral budding (Li et al., 2003 [PubMed 14635195]; Amit et al., 2004 [PubMed 15256501]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LRSAM1