Home » Chp2 » Chp2 Lysates » Chp2 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Chp2 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Chp2 Overexpression Lysate (Adult Normal) [NBP2-06467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chp2.
Western Blot: Chp2 Overexpression Lysate (Adult Normal) [NBP2-06467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chp2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Chp2 Overexpression Lysate Summary

Description

Chp2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC212776

Accession#: NM_022097

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CHP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Chp2 Overexpression Lysate

  • calcineurin B homologous protein 2
  • HCA520
  • hepatocellular carcinoma antigen gene 520
  • Hepatocellular carcinoma-associated antigen 520

Background

Chp2 binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cellsfrom serum deprivation-induced death

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...


Calcium-binding-protei
NBP1-33736
Immunohistochemistry-Paraffin Calcium-binding-protein-P22 Antibody
Immunocytochemistry/ Immunofluorescence Calcium-binding-protein-P22 Antibody
Immunohistochemistry-Paraffin Calcium-binding-protein-P22 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
RhoV Antibody
NBP2-49082
Immunohistochemistry-Paraffin RhoV Antibody
Immunohistochemistry-Paraffin RhoV Antibody
Immunohistochemistry-Paraffin RhoV Antibody
Species: Hu
Applications: IHC, IHC-P
CHORDC1 Antibody -
BSA Free
NBP1-78304
Immunocytochemistry/ Immunofluorescence CHORDC1 Antibody - BSA Free
Immunohistochemistry CHORDC1 Antibody - BSA Free
Western Blot CHORDC1 Antibody - BSA Free
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NHE1/SLC9A1
Antibody - BSA Free
NBP1-76847
Immunocytochemistry/ Immunofluorescence NHE1/SLC9A1 Antibody - BSA Free
Western Blot NHE1/SLC9A1 Antibody - BSA Free
Immunohistochemistry-Paraffin NHE1/SLC9A1 Antibody - BSA Free
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
tescalcin Antibody
NBP2-13426
Immunocytochemistry/ Immunofluorescence tescalcin Antibody
Immunohistochemistry-Paraffin tescalcin Antibody
Immunohistochemistry-Paraffin tescalcin Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HP1 alpha Antibody
NB100-2518
Flow (Intracellular) HP1 alpha Antibody
Immunocytochemistry/ Immunofluorescence HP1 alpha Antibody
Western Blot HP1 alpha Antibody
Species: Hu
Applications: Flow-IC, ICC/IF, WB
alpha-Defensin 1
[Biotin]
DY8198-05
N/A alpha-Defensin 1 [Biotin]
(5 Publications)
Species: Hu
Applications: ELISA
Histone H3
Antibody - BSA Free
NB500-171
Immunohistochemistry-Paraffin Histone H3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Histone H3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Histone H3 Antibody - BSA Free
(8 Reviews)
(34 Publications)
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
GLI-2 Antibody
AF3635
Immunohistochemistry GLI-2 Antibody
(2 Reviews)
(43 Publications)
Species: Mu
Applications: IHC, WB
HDAC9 Antibody -
BSA Free
NBP2-03993
Western Blot HDAC9 Antibody - BSA Free
(1 Review)
(1 Publication)
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB
Melanoma antigen
family C2 Antibody
NBP2-34145
Immunohistochemistry-Paraffin Melanoma antigen family C2 Antibody
Immunohistochemistry-Paraffin Melanoma antigen family C2 Antibody
Immunohistochemistry-Paraffin Melanoma antigen family C2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TPX2 Antibody
NB500-179
Flow Cytometry TPX2 Antibody
Simple Western TPX2 Antibody
Immunocytochemistry/ Immunofluorescence TPX2 Antibody
(1 Review)
(47 Publications)
Species: Hu, Ma, Mu, Po, Rt
Applications: Flow, ICC/IF, IP, KD, Simple Western, WB
FAM126A Antibody
NBP1-32914
Western Blot FAM126A Antibody
Western Blot FAM126A Antibody
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, WB
NFATC3/NFAT4
Antibody [Unconjugate
AF5834
Western Blot NFATC3/NFAT4 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(650 Publications)
Species: Mu
Applications: ELISA
Osteopontin/OPN
Antibody [Unconjugate
AF808
Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]
Neutralization Osteopontin/OPN Antibody [Unconjugated]
Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]
(2 Reviews)
(205 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
Contactin-3
Antibody [Unconjugate
AF5539
Western Blot Contactin-3 Antibody [Unconjugated]
Immunocytochemistry Contactin-3 Antibody [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
CENPA Antibody
NBP3-13389
Western Blot CENPA Antibody
Immunohistochemistry-Paraffin CENPA Antibody
Species: Hu
Applications: IHC, IHC-P, WB

Publications for Chp2 Lysate (NBP2-06467) (0)

There are no publications for Chp2 Lysate (NBP2-06467).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Chp2 Lysate (NBP2-06467) (0)

There are no reviews for Chp2 Lysate (NBP2-06467). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Chp2 Lysate (NBP2-06467) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Chp2 Products

Array NBP2-06467

Blogs on Chp2

There are no specific blogs for Chp2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Osteopontin/OPN Antibody [Unconjugated]
AF808
Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly positive for both RHAMM and the intracellular positive control protein TPX2 (right panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0073548) licensed under a CC-BY license.
TPX2 Antibody
NB500-179

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Chp2 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol CHP2