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Chk1 [p Ser280] Antibody - Azide and BSA Free

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Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more
Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded mouse brain using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded rat heart using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded human placenta using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-05379-20ul
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Chk1 [p Ser280] Antibody - Azide and BSA Free Summary

Immunogen
A synthetic phosphorylated peptide around S280 of human Chk1 (NP_001265.2). VTSGG
Modification
p Ser280
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Chk1 [p Ser280] Antibody - Azide and BSA Free

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHEK1