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CHAF1B Antibody (SS 24 1-68)

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Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using NB500-211.
Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using NB500-211.
Immunocytochemistry/ Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SS 24 1-68
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CHAF1B Antibody (SS 24 1-68) Summary

Immunogen
Recombinant human CHAF1B [UniProt# Q13112]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHAF1B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 0.1-1 ug
  • Western Blot 1:1000
Application Notes
This CHAF1B (SS 24 1-68) antibody is useful for Immunoprecipitation, Western blot and Immunocytochemistry/Immunofluorescence. In WB a band can be seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CHAF1B Overexpression Lysate
Publications
Read Publications using
NB500-211 in the following applications:

  • ICC/IF
    1 publication
  • IP
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for CHAF1B Antibody (SS 24 1-68)

  • CAF1
  • CAF-1
  • CAF1ACAF-1 subunit B
  • CAF1P60CAF-I 60 kDa subunit
  • CAF-IP60
  • chromatin assembly factor 1 subunit B
  • chromatin assembly factor 1, subunit B (p60)
  • Chromatin assembly factor I p60 subunit
  • human chromatin assembly factor-I p60 subunit, 10
  • MPHOSPH7CAF-I p60
  • MPP7M-phase phosphoprotein 7

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CHAF1B Antibody
(SS 24 1-68)
NB500-211
CHAF1B Antibody (SS 24 1-68)
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IP

Publications for CHAF1B Antibody (NB500-211)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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(1)
IP
(2)
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(1)
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Product General Protocols

View specific protocols for CHAF1B Antibody (NB500-211): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHAF1B Antibody (NB500-211) (0)

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Control Lysate(s)

CHAF1B Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHAF1B
Entrez
Uniprot