![Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Western-Blot-NBP2-12892-img0002.jpg "Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 5A7-2E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A |
| Localization | Nucleus , Chromosome , Centromere , Kinetochore |
| Marker | Centromeres Marker |
| Specificity | Detects approx 18kDa. Recognizes Human CENP-A. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CENPA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of CENP-A Antibody was sufficient for detection of CENPA in 20 ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CENPA |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Immunocytochemistry-Immunofluorescence-NBP2-12892-img0003.jpg "Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")