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CDT1 Antibody

Images

 
Western Blot: CDT1 Antibody [NBP1-58114] - Sample Type: 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000
Immunocytochemistry/ Immunofluorescence: CDT1 Antibody [NBP1-58114] - Mouse brain stem cells, 2 ug/ml.
Western Blot: CDT1 Antibody [NBP1-58114] - Sample Type : 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody : IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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CDT1 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to CDT1(chromatin licensing and DNA replication factor 1) The peptide sequence was selected from the C terminal of CDT1. Peptide sequence PATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQ. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDT1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for CDT1 Antibody

  • chromatin licensing and DNA replication factor 1
  • DNA replication factor Cdt1
  • Double parked homolog
  • Double parked, Drosophila, homolog of
  • DUPRIS2

Background

CDT1 cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It binds DNA in a sequence-, strand-, and conformation-independent manner and is a potential oncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDT1 Antibody (NBP1-58114) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDT1 Antibody (NBP1-58114). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody work on mouse tissue with immunofluorescent protocols? Also which fixation method is recommended? Finally, is there any warranty for the antibody that will cover our purchase with a full refund in case this product does not work?
    • All of our products are 100% guaranteed for the applications and species listed on the datasheet. In the case of NBP1-58114, it is guaranteed for use in Western blot and immunocytochemistry on human, mouse and rat samples. The image showing staining in mouse brain came from a customer. I have the following information regarding their protocol. Primary antibody dilution: 1:500, Secondary antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary antibody dilution: 1:1000, IHC Protocol: Incubate cells with 2N HCL for 30 min, Wash 3X in PBS, Stained for 1h 30 min with 1:500 antibody, Wash 3X in PBS, Incubated with goat anti-rabbit Alexa-Fluor 594 for 1h, Wash 3X in PBS, Co stain with DAPI, Images were taken with a 40X objective.

Secondary Antibodies

 

Isotype Controls

Additional CDT1 Products

Array NBP1-58114

Research Areas for CDT1 Antibody (NBP1-58114)

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Blogs on CDT1

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Bioinformatics

Gene Symbol CDT1