CDT1 Antibody Summary
Description |
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
Immunogen |
Synthetic peptides corresponding to CDT1(chromatin licensing and DNA replication factor 1) The peptide sequence was selected from the C terminal of CDT1. Peptide sequence PATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQ. The peptide sequence for this immunogen was taken from within the described region. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CDT1 |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:10-1:2000
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin
- Western Blot 1.0 ug/ml
|
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS, 2% Sucrose |
Preservative |
0.09% Sodium Azide |
Concentration |
0.5 mg/ml |
Purity |
Affinity purified |
Alternate Names for CDT1 Antibody
Background
CDT1 cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It binds DNA in a sequence-, strand-, and conformation-independent manner and is a potential oncogene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for CDT1 Antibody (NBP1-58114) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for CDT1 Antibody (NBP1-58114). (Showing 1 - 1 of 1 FAQ).
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Does this antibody work on mouse tissue with immunofluorescent protocols? Also which fixation method is recommended? Finally, is there any warranty for the antibody that will cover our purchase with a full refund in case this product does not work?
- All of our products are 100% guaranteed for the applications and species listed on the datasheet. In the case of NBP1-58114, it is guaranteed for use in Western blot and immunocytochemistry on human, mouse and rat samples. The image showing staining in mouse brain came from a customer. I have the following information regarding their protocol. Primary antibody dilution: 1:500, Secondary antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary antibody dilution: 1:1000, IHC Protocol: Incubate cells with 2N HCL for 30 min, Wash 3X in PBS, Stained for 1h 30 min with 1:500 antibody, Wash 3X in PBS, Incubated with goat anti-rabbit Alexa-Fluor 594 for 1h, Wash 3X in PBS, Co stain with DAPI, Images were taken with a 40X objective.
Secondary Antibodies
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Isotype Controls
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