Home » CDCA5 » CDCA5 Lysates » CDCA5 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

CDCA5 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: CDCA5 Overexpression Lysate (Adult Normal) [NBL1-09019] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDCA5.
Western Blot: CDCA5 Overexpression Lysate (Adult Normal) [NBL1-09019] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDCA5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CDCA5 Overexpression Lysate Summary

Description

CDCA5 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203624

Accession#: NM_080668

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CDCA5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
27.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CDCA5 Overexpression Lysate

  • cell division cycle associated 5
  • Cell division cycle-associated protein 5
  • MGC16386
  • p35
  • SORORIN

Background

Regulator of sister chromatid cohesion in mitosis. May act by regulating the ability of the cohesin complexto mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with thechromosomes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cdk5 Antibody
NBP2-15843
Western Blot Cdk5 Antibody
Immunohistochemistry-Paraffin Cdk5 Antibody
Immunocytochemistry/ Immunofluorescence Cdk5 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
CDK5 Activator 1
Antibody
NBP3-13279
Immunocytochemistry/ Immunofluorescence CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
p63/TP73L Antibody
[Unconjugated]
AF1916
Immunohistochemistry p63/TP73L Antibody [Unconjugated]
Immunocytochemistry p63/TP73L Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence p63/TP73L Antibody [Unconjugated]
(1 Review)
(24 Publications)
Species: Hu
Applications: ICC, IHC, WB
IL-9 [Unconjugated]
409-ML
IL-9 [Unconjugated]
(13 Publications)
Species: Mu
Applications: BA
GEF-H1 Antibody
NBP2-21577
Western Blot GEF-H1 Antibody
Western Blot GEF-H1 Antibody
Western Blot GEF-H1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
PSMD7 Antibody
NBP2-38780
Immunocytochemistry/ Immunofluorescence PSMD7 Antibody
Immunohistochemistry-Paraffin PSMD7 Antibody
Western Blot PSMD7 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
EBNA1BP2 Antibody
NBP1-84022
Immunocytochemistry/ Immunofluorescence EBNA1BP2 Antibody
Western Blot EBNA1BP2 Antibody
Western Blot EBNA1BP2 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
LANCL1 Antibody
NBP1-81796
Immunocytochemistry/ Immunofluorescence LANCL1 Antibody
Immunohistochemistry-Paraffin LANCL1 Antibody
Immunohistochemistry-Paraffin LANCL1 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
p40 Antibody
NBP1-32956
Immunocytochemistry/ Immunofluorescence p40 Antibody
Immunohistochemistry-Paraffin p40 Antibody
Western Blot p40 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
RPSA Antibody
(RPSA/2699) - Azide a ...
NBP2-79854
Immunohistochemistry-Paraffin RPSA Antibody (RPSA/2699) - Azide and BSA Free
Immunohistochemistry-Paraffin RPSA Antibody (RPSA/2699) - Azide and BSA Free
Immunohistochemistry-Paraffin RPSA Antibody (RPSA/2699) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, PA, WB
TPPP/p25 Antibody
NBP2-34031
Western Blot TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TMED9 Antibody
(A9A8)
NBP3-33026
Western Blot TMED9 Antibody (A9A8)
Immunohistochemistry TMED9 Antibody (A9A8)
Immunocytochemistry/ Immunofluorescence TMED9 Antibody (A9A8)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Lipocalin-2/NGAL
[Biotin]
DY1857
N/A Lipocalin-2/NGAL [Biotin]
(132 Publications)
Species: Mu
Applications: ELISA
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(452 Publications)
Species: Hu
Applications: BA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
Tau Antibody (2E9)
NBP2-25162
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
Western Blot Tau Antibody (2E9)
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
(5 Publications)
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(388 Publications)
Species: Hu, Mu
Applications: ICC, IHC
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB

Publications for CDCA5 Lysate (NBL1-09019) (0)

There are no publications for CDCA5 Lysate (NBL1-09019).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CDCA5 Lysate (NBL1-09019) (0)

There are no reviews for CDCA5 Lysate (NBL1-09019). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CDCA5 Lysate (NBL1-09019) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CDCA5 Products

Research Areas for CDCA5 Lysate (NBL1-09019)

Find related products by research area.

Blogs on CDCA5

There are no specific blogs for CDCA5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
IFN-gamma [Unconjugated]
285-IF-100

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CDCA5 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol CDCA5