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CD53 Antibody (MEM-53) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
MEM-53
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-393AF350
Alexa Fluor 405
NB500-393AF405
Alexa Fluor 488
NB500-393AF488
Alexa Fluor 532
NB500-393AF532
Alexa Fluor 594
NB500-393AF594
Alexa Fluor 647
NB500-393AF647
Alexa Fluor 700
NB500-393AF700
Alexa Fluor 750
NB500-393AF750
Allophycocyanin
NB500-393APC
Allophycocyanin/Cy7
NB500-393APCCY7
Biotin
NB500-393B
CoraFluor 1
NB500-393CL1
DyLight 350
NB500-393UV
DyLight 405
NB500-393V
DyLight 488
NB500-393G
DyLight 550
NB500-393R
DyLight 594
NB500-393DL594
DyLight 650
NB500-393C
DyLight 680
NB500-393FR
DyLight 755
NB500-393IR
FITC
NB500-393F
HRP
NB500-393H
Janelia Fluor 525
NB500-393JF525
Janelia Fluor 549
NB500-393JF549
Janelia Fluor 585
NB500-393JF585
Janelia Fluor 646
NB500-393JF646
Janelia Fluor 669
NB500-393JF669
PE
NB500-393PE
PE/Atto594
NB500-393PEATT594
PE/Cy5.5
NB500-393PECY55
PE/Cy7
NB500-393PECY7
PerCP
NB500-393PCP
Unconjugated
NB500-393
mFluor Violet 450 SE
NB500-393MFV450
mFluor Violet 500 SE
NB500-393MFV500
mFluor Violet 610 SE
NB500-393MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-393
BSA Free
NB500-393
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CD53 Antibody (MEM-53) [Janelia Fluor® 635] Summary

Additional Information
Clone MEM-53 was used by HLDA to establish CD designation.
Immunogen
Leukocytes of patient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD53
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD53 Antibody (MEM-53) [Janelia Fluor® 635]

  • antigen MOX44 identified by monoclonal MRC-OX44
  • CD53 antigentetraspanin-25
  • CD53 glycoprotein
  • CD53 molecule
  • CD53 tetraspan antigen
  • CD53
  • cell surface antigen
  • Cell surface glycoprotein CD53
  • MOX44
  • MOX44transmembrane glycoprotein
  • Tetraspanin-25
  • TSPAN25
  • tspan-25
  • TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD53