![Western Blot: CD45RA Antibody (MEM-56) [NB500-329] - Fig. 1. Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD4 degrees Control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.](http://images.novusbio.com/fullsize/CD45RA-Antibody-MEM-56-Western-Blot-NB500-329-img0002.jpg "Western Blot: CD45RA Antibody (MEM-56) [NB500-329] - Fig. 1. Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD4 degrees Control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IHC, IP, CyTOF-ready |
| Clone | MEM-56 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone MEM-56 was used by HLDA to establish CD designation. |
| Immunogen | Human thymocytes and T lymphocytes. |
| Specificity | The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes. HLDA IV; WS Code NL 907 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTPRC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry-Paraffin sections: No pre-treatment of tissue sections is essential. This antibody is CyTOF ready. |
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| Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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| Publication using NB500-329 | Applications | Species |
|---|---|---|
| Jordi CA Development of Proximity Ligation Assay variants for single cell proteomics Thesis 2020-01-01 (PLA) | PLA |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD45RA Antibody (NB500-329)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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