CD45RA Antibody (1069433) [Unconjugated] Summary
Immunogen |
Whole cells (naïve T cells) Accession # P08575 |
Specificity |
Detects CD45RA in direct ELISA. |
Source |
N/A |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25 ug/10^6 cells
- Immunohistochemistry 5-25 ug/mL
- Multiplex Immunofluorescence 2.5 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD45RA Antibody (1069433) [Unconjugated]
Background
This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA. CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene. In humans, CD45RA is one of six isoforms of the CD45 complex. These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC. CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells. CD45RA contains exon 4 but lacks both exons 5 and 6. CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons. Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells. Other cell types such as activated monocytes and B cells can also express CD45RO. Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Species: Rt
Applications: IHC, WB
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow, WB
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
Applications: Dual ISH-IHC, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
Species: Mu
Applications: ELISA
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, In vitro, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IP, KO
Publications for CD45RA Antibody (MAB11444) (0)
There are no publications for CD45RA Antibody (MAB11444).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for CD45RA Antibody (MAB11444) (0)
There are no reviews for CD45RA Antibody (MAB11444).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for CD45RA Antibody (MAB11444) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional CD45RA Products
Blogs on CD45RA