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Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

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ELISA: Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP2-75151] - Samples were spiked with high concentrations of Mouse CD40 Ligand/TNFSF5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP2-75151] - Samples were spiked with high concentrations of Mouse CD40 Ligand/TNFSF5 and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP2-75151] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
93.75 - 6000 pg/mL
Sensitivity
56.25 pg/mL

Order Details

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Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse CD40L in samples. No significant cross-reactivity or interference between Mouse CD40L and analogues was observed.
Standard Curve Range
93.75 - 6000 pg/mL
Sensitivity
56.25 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.14%
Intra-Assay
CV% < 4.84%
Spike Recovery
85-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD40LG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40 Ligand/TNFSF5 ELISA Kit (NBP2-75151). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Contact Information

Product PDFs

  SDS
  Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol CD40LG