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Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

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Mouse CD40 Ligand/TNFSF5 ELISA Kit [NBP1-92662] - Data for Mouse sCD40L Platinum ELISA

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
0.31 - 20.0 ng/mL
Sensitivity
0.14 ng/mL

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Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) Summary

Description
The mouse sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse sCD40L
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse CD40L positive serum. There was no crossreactivity detected.
Standard Curve Range
0.31 - 20.0 ng/mL
Sensitivity
0.14 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 11.10%
Intra-Assay
CV% < 6.50%
Spike Recovery
The overall mean recovery was 85%. Recoveries were shown to depend on the serum used. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD40LG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
  5. Mouse sCD40L Standard lyophilized, 40 ng/ml upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

The murine cell surface antigen is known also as Ly62. CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CD40 Ligand/TNFSF5 ELISA Kit (NBP1-92662). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Bioinformatics

Gene Symbol CD40LG