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CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

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Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.
Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.
Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in Analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
MR-1
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-47234AF350
Alexa Fluor 532
NBP1-47234AF532
Alexa Fluor 594
NBP1-47234AF594
Alexa Fluor 700
NBP1-47234AF700
Alexa Fluor 750
NBP1-47234AF750
DyLight 594
NBP1-47234DL594
mFluor Violet 450 SE
NBP1-47234MFV450
mFluor Violet 500 SE
NBP1-47234MFV500
mFluor Violet 610 SE
NBP1-47234MFV610
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CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free Summary

Immunogen
Activated mouse Th1 clone D1.6
Specificity
The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD40LG
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:50
Application Notes
Functional Application: Blocking T cell costimulation in vivo. This antibody is CyTOF ready.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD40 Ligand/TNFSF5 Antibody (NBP1-47234). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD40LG