![Immunohistochemistry-Paraffin: CD35 Antibody (SPM554) - IHC-Prediluted [NBP2-44618] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (SPM554).](http://images.novusbio.com/fullsize/CD35-Antibody-SPM554-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44618-img0001.jpg "Immunohistochemistry-Paraffin: CD35 Antibody (SPM554) - IHC-Prediluted [NBP2-44618] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (SPM554).")
| Reactivity | Hu, Pm, Pm-Cm, RMSpecies Glossary |
| Applications | IHC |
| Clone | SPM554 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Intact human monocytes |
| Localization | Cell surface |
| Marker | Follicular Dendritic Cell Marker |
| Specificity | Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CR1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD35 Antibody (NBP2-44618)Find related products by research area.
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How To Identify B Cell Subsets Using Flow Cytometry By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified... Read full blog post. |
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| Gene Symbol | CR1 |
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