![Immunohistochemistry: CD35 Antibody (7G6) - Chimeric [NBP2-52667] - Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody NBP2-52667. Formalin fixed rat stomach slices were were stained with NBP2-52667 at 3 ug/ml.](http://images.novusbio.com/fullsize/CD35-Antibody-7G6-Chimeric-Immunohistochemistry-NBP2-52667-img0001.jpg "Immunohistochemistry: CD35 Antibody (7G6) - Chimeric [NBP2-52667] - Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody NBP2-52667. Formalin fixed rat stomach slices were were stained with NBP2-52667 at 3 ug/ml.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, Flow, Func, IHC, IP, B/N, CyTOF-ready |
| Clone | 7G6 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Purified Mouse CR1. |
| Specificity | Recognises an epitope shared by 145-150kDa and 190kDa complement receptor proteins, originally designated CR2 (CD21) and CR1 (CD35). |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. This antibody binds mouse complement receptors CR1 and CR2, functionally blocking CR2 leading to suppression of a normal antibody immune response (see Heyman et al. 1990, PMID: 1695671) |
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| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD35 Antibody (NBP2-52667)Find related products by research area.
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How To Identify B Cell Subsets Using Flow Cytometry By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified... Read full blog post. |
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| Gene Symbol | CR1 |
![Flow Cytometry: CD35 Antibody (7G6) - Chimeric [NBP2-52667] - Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 (NBP2-52667). Mouse lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 7G6 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD35-Antibody-7G6-Chimeric-Flow-Cytometry-NBP2-52667-img0002.jpg "Flow Cytometry: CD35 Antibody (7G6) - Chimeric [NBP2-52667] - Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 (NBP2-52667). Mouse lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 7G6 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")