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Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

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ELISA: Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) [NBP2-82140] - Samples were spiked with high concentrations of Rat CD30/TNFRSF8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) [NBP2-82140] - Samples were spiked with high concentrations of Rat CD30/TNFRSF8 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) [NBP2-82140] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

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Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CD30/TNFRSF8 in samples. No Significant cross-reactivity or interference between Rat CD30/TNFRSF8 and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 5%
Spike Recovery
average = 91% for serum; average = 97% for EDTA plasma; average = 97% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF8

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

  • CD30 antigen
  • CD30
  • CD30KI-1
  • CD30L receptor
  • cytokine receptor CD30
  • D1S166EKi-1
  • Ki-1 antigen
  • Lymphocyte activation antigen CD30
  • TNFRSF8
  • tumor necrosis factor receptor superfamily member 8
  • tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol TNFRSF8