Home » CD30/TNFRSF8 » CD30/TNFRSF8 Antibodies » CD30/TNFRSF8 Antibody (Ki-3) [CoraFluor™ 1]
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CD30/TNFRSF8 Antibody (Ki-3) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, Func, ICC/IF, IHC, CyTOF-ready
Clone
Ki-3
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-52682AF350
Alexa Fluor 405
NBP2-52682AF405
Alexa Fluor 488
NBP2-52682AF488
Alexa Fluor 532
NBP2-52682AF532
Alexa Fluor 594
NBP2-52682AF594
Alexa Fluor 647
NBP2-52682AF647
Alexa Fluor 700
NBP2-52682AF700
Alexa Fluor 750
NBP2-52682AF750
Allophycocyanin
NBP2-52682APC
Biotin
NBP2-52682B
DyLight 350
NBP2-52682UV
DyLight 405
NBP2-52682V
DyLight 488
NBP2-52682G
DyLight 550
NBP2-52682R
DyLight 594
NBP2-52682DL594
DyLight 650
NBP2-52682C
DyLight 680
NBP2-52682FR
DyLight 755
NBP2-52682IR
FITC
NBP2-52682F
HRP
NBP2-52682H
Janelia Fluor 525
NBP2-52682JF525
Janelia Fluor 549
NBP2-52682JF549
Janelia Fluor 585
NBP2-52682JF585
Janelia Fluor 635
NBP2-52682JF635
Janelia Fluor 646
NBP2-52682JF646
Janelia Fluor 669
NBP2-52682JF669
PE
NBP2-52682PE
PerCP
NBP2-52682PCP
Unconjugated
NBP2-52682-0.2mg
mFluor Violet 450 SE
NBP2-52682MFV450
mFluor Violet 500 SE
NBP2-52682MFV500
mFluor Violet 610 SE
NBP2-52682MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-52682-0.2mg
Azide and BSA Free
NBP2-52682-0.2mg
Azide and BSA Free
NBP2-52682-0.025mg
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CD30/TNFRSF8 Antibody (Ki-3) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human CD30.
Specificity
The antibody binds to CD30.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
TNFRSF8
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD30/TNFRSF8 Antibody (Ki-3) [CoraFluor™ 1]

  • CD30 antigen
  • CD30
  • CD30KI-1
  • CD30L receptor
  • cytokine receptor CD30
  • D1S166EKi-1
  • Ki-1 antigen
  • Lymphocyte activation antigen CD30
  • TNFRSF8
  • tumor necrosis factor receptor superfamily member 8
  • tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF8