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CD30/TNFRSF8 Antibody (81337) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, Func
Clone
81337
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB229U-100UG
Alexa Fluor 405
FAB229V-100UG
Alexa Fluor 488
FAB229G-100UG
Alexa Fluor 532
FAB229X
Alexa Fluor 594
FAB229T-100UG
Alexa Fluor 647
FAB229R-100UG
Alexa Fluor 700
FAB229N-100UG
Alexa Fluor 750
FAB229S-100UG
Allophycocyanin
FAB229A
Allophycocyanin/Cy7
FAB229APCCY7
Biotin
FAB229B
DyLight 350
FAB229D
DyLight 405
FAB229E
DyLight 488
FAB229K
DyLight 550
FAB229L
DyLight 594
FAB229M
DyLight 650
FAB229W
DyLight 680
FAB229Y
DyLight 755
FAB229Z
HRP
FAB229H
Janelia Fluor 549
FAB229I
Janelia Fluor 585
FAB229JF585
Janelia Fluor 635
FAB229JF635
Janelia Fluor 646
FAB229J
Janelia Fluor 669
FAB229JF669
PE/Atto594
FAB229PEATT594
PE/Cy5.5
FAB229PECY55
PE/Cy7
FAB229PECY7
PerCP
FAB229C
Unconjugated
MAB229
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB229
Carrier Free
MAB229
Carrier Free
MAB229-SP
Carrier Free
FAB229V-100UG
Carrier Free
FAB229U-100UG
Carrier Free
FAB229T-100UG
Carrier Free
FAB229S-100UG
Carrier Free
FAB229R-100UG
Carrier Free
FAB229N-100UG
Carrier Free
FAB229G-100UG
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CD30/TNFRSF8 Antibody (81337) [Janelia Fluor® 525] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD30/TNFRSF8
Phe19-Lys379
Accession # P28908
Specificity
Detects human CD30/TNFRSF8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD30, recombinant human (rh) CD27, and rhCD40 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF8
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Agonist Activity
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD30/TNFRSF8 Antibody (81337) [Janelia Fluor® 525]

  • CD30 antigen
  • CD30
  • CD30KI-1
  • CD30L receptor
  • cytokine receptor CD30
  • D1S166EKi-1
  • Ki-1 antigen
  • Lymphocyte activation antigen CD30
  • TNFRSF8
  • tumor necrosis factor receptor superfamily member 8
  • tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF8