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CD19 Antibody (4G7-2E3) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD19 Monoclonal Antibody ...read more
Common heavy-chain antibodies are specific and have neutralization potential.(a) Flow cytometry profiles obtained with different IgGs selected against CD19, EpCAM, EGFR and CD47. The profiles on antigen-negative versus ...read more
Biological activity of bispecific kappa lambda -bodies.(a) Schematic representation of monovalent and bivalent binding of CD19xCD47 kappa lambda -bodies on the surface of CD47+/CD19−DS-1 cells (top) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
4G7-2E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CD19 Antibody (4G7-2E3) [Unconjugated] Summary

Immunogen
Human CLL cells
Specificity
Detects human CD19.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB4867 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD19 Antibody (4G7-2E3) [Unconjugated]

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD19 Antibody (MAB4867)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(5)
All Applications
Filter By Species
Human
(4)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB4867 Applications Species
Morten Hjuler Nielsen, Rikke Bæk, Malene Moller Jorgensen, Maiken Mellergaard, Aase Handberg Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia Atherosclerosis Plus 2023-09-01 [PMID: 37637934]
SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019-02-01;0(0):. 2019-02-01 [PMID: 30720108] (Flow Cytometry, Rat) Flow Cytometry Rat
U Ellinghaus, A Cortini, CL Pinder, GL Friec, C Kemper, TJ Vyse Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus Eur. J. Immunol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28444759] (Flow Cytometry, Human) Flow Cytometry Human
Elie Dheilly, Valéry Moine, Lucile Broyer, Susana Salgado-Pires, Zoë Johnson, Anne Papaioannou et al. Selective Blockade of the Ubiquitous Checkpoint Receptor CD47 Is Enabled by Dual-Targeting Bispecific Antibodies Molecular Therapy 2017-02-01 [PMID: 28153099]
Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
Fischer N, Elson G, Magistrelli G, Dheilly E, Fouque N, Laurendon A, Gueneau F, Ravn U, Depoisier J, Moine V, Raimondi S, Malinge P, Di Grazia L, Rousseau F, Poitevin Y, Calloud S, Cayatte P, Alcoz M, Pontini G, Fagete S, Broyer L, Corbier M, Schrag D, Didelot G, Bosson N, Costes N, Cons L, Buatois V, Johnson Z, Ferlin W, Masternak K, Kosco-Vilbois M Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun, 2015-02-12;6(0):6113. 2015-02-12 [PMID: 25672245] (Flow Cytometry, Human) Flow Cytometry Human
Lee F, Luevano M, Veys P, Yong K, Madrigal A, Shaw B, Saudemont A The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface. PLoS ONE, 2014-07-22;9(7):e103254. 2014-07-22 [PMID: 25050704] (Flow Cytometry, Human) Flow Cytometry Human
Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-kappaB pathway. J. Cell. Sci., 2012-03-29;91(6):933-45. 2012-03-29 [PMID: 22459945] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

Isotype Controls

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How To Identify B Cell Subsets Using Flow Cytometry
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Antigen-loss relapse after successful CAR-T therapy; What do we do now?
By Jacqueline Carrico, BS, MD Tumor cell mechanisms driving tolerance to CAR-T Despite very promising results of CAR-T therapy in acute lymphoblastic leukemia, B-ALL, antigen-loss relapse has arisen as a major chall...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19