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CD163 Antibody (612722) [Unconjugated]

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Western Blot shows lysates of mouse liver. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD163 at approximately 140 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Western Blot shows lysates of mouse liver. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
CD163 was detected in fixed Neuro2A cells (Positive) using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
612722
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7435-SP
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CD163 Antibody (612722) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line, NS0-derived recombinant mouse CD163
Val39-Thr1045
Accession # Q2VLH6
Specificity
Detects mouse CD163 in direct ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB7435 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD163 Antibody (612722) [Unconjugated]

  • CD_antigen: CD163
  • CD163 molecule
  • CD163
  • GHI/61
  • HbSR
  • Hemoglobin scavenger receptor
  • M130
  • macrophage-associated antigen
  • MM130
  • RM3/1
  • SCARI1
  • scavenger receptor cysteine-rich type 1 protein M130
  • sCD163
  • Soluble CD163

Background

CD163, also known as M130 and  p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).
  1. Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
  2. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  3. Hogger, P. et al. (1998) J. Immunol. 161:1883.
  4. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  5. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  6. Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
  7. Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
  8. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  9. Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  10. Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
  11. Fabriek, B.O. et al. (2009) Blood 113:887.
  12. Kristiansen, M. et al. (2001) Nature 409:198.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Fabriek, B.O. et al. (2007) Blood 109:5223.
  15. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  16. Moreno, J.A. et al. (2009) Atherosclerosis 207:103.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD163 Antibody (MAB7435)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FACS.


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