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CD14 Antibody

Images

 
Independent Antibodies: Western Blot: CD14 Antibody [NBP1-86166] - Analysis using Anti-CD14 antibody NBP1-86166 (A) shows similar pattern to independent antibody NBP1-86168 (B).
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.
Independent Antibodies: Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86166 (A) shows similar protein ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD14 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPACARSTLSVGVSGTLVLLQG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC-P reported in scientific literature (PMID: 30049749). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CD14 Protein (NBP1-86166PEP)
Publications
Read Publications using
NBP1-86166 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24743550)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD14 Antibody

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NBP1-86166)(3)

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Product General Protocols

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WB Video Protocol

FAQs for CD14 Antibody (NBP1-86166). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in your anti-human CD14 (NBP1-86166). What concentration does this antibodies has? Does it come in carrier-free form (without protein stabilizer/BSA)?
    • Our product NBP1-86166 is supplied in PBS (pH 7.2) with 40% glycerol and 0.02% sodium azide. The current lot has a concentration of 0.4mg/ml.

Secondary Antibodies

 

Isotype Controls

Additional CD14 Products

Research Areas for CD14 Antibody (NBP1-86166)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol CD14