Home » CD117/c-kit » CD117/c-kit ELISA Kits » Human CD117/c-kit ELISA Kit (Colorimetric)
Novus Biologicals products are now on bio-techne.com

Human CD117/c-kit ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Human CD117/c-kit ELISA Kit (Colorimetric) [NBP3-14627] - Example of Human CD117/c-kit Standard Curve.
ELISA: Human CD117/c-kit ELISA Kit (Colorimetric) [NBP3-14627] - Example of Human CD117/c-kit Standard Curve.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell culture supernatant
Standard Curve Range
0.312-10 ng/ml
Sensitivity
0.095 ng/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human CD117/c-kit ELISA Kit (Colorimetric) Summary

Description
Incubation: 1 hours 45 minutes
Specificity
Recognizes both natural and recombinant human soluble CD117/c-kit. No cross reactivity with other human soluble receptors.
Standard Curve Range
0.312-10 ng/ml
Sensitivity
0.095 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.9
Intra-Assay
CV% < 6
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
KIT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. Biotinylated secondary antibody
  2. Buffers
  3. Controls (where applicable)
  4. Detailed procedure.
  5. Pre-coated strip plates
  6. Standards
  7. Stop reagents
  8. Streptavidin-HRP
  9. TMB

Alternate Names for Human CD117/c-kit ELISA Kit (Colorimetric)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

SCF/c-kit Ligand
[Unconjugated]
255-SC
SCF/c-kit Ligand [Unconjugated]
(203 Publications)
Species: Hu
Applications: BA
CD34 Antibody
[Unconjugated]
AF4117
Immunohistochemistry CD34 Antibody [Unconjugated]
(3 Reviews)
(37 Publications)
Species: Rt
Applications: IHC, WB
PDGFR alpha
Antibody [Unconjugate
AF1062
Western Blot PDGFR alpha Antibody [Unconjugated]
Immunohistochemistry PDGFR alpha Antibody [Unconjugated]
Neutralization PDGFR alpha Antibody [Unconjugated]
(4 Reviews)
(309 Publications)
Species: Mu
Applications: Flow, IHC, Neut, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(821 Publications)
Species: Hu
Applications: BA
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(389 Publications)
Species: Hu, Mu
Applications: ICC, IHC
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(676 Publications)
Species: Mu
Applications: ELISA
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Flt-3/Flk-2/CD135
Antibody (66903) [Unc ...
MAB812
Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]
Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
Desmin Antibody
[Unconjugated]
AF3844
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: IHC
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(219 Publications)
Species: Hu
Applications: BA
Vimentin Antibody
NB300-223
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
Immunohistochemistry-Frozen Vimentin Antibody
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
(6 Reviews)
(80 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
CD45 Antibody
[Unconjugated]
AF114
Immunohistochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]
(1 Review)
(72 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(43 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
PDGF R beta
Antibody [Unconjugate
AF1042
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
Western Blot PDGF R beta Antibody [Unconjugated]
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
(110 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
Human CD117/c-kit
ELISA Kit (Colorimetr ...
NBP3-14627
Human CD117/c-kit ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

Publications for CD117/c-kit ELISA Kit (NBP3-14627) (0)

There are no publications for CD117/c-kit ELISA Kit (NBP3-14627).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD117/c-kit ELISA Kit (NBP3-14627) (0)

There are no reviews for CD117/c-kit ELISA Kit (NBP3-14627). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD117/c-kit ELISA Kit (NBP3-14627) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CD117/c-kit Products

Research Areas for CD117/c-kit ELISA Kit (NBP3-14627)

Find related products by research area.

Blogs on CD117/c-kit.

Check out the latest blog posts on CD117/c-kit.
Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165&nbsp;kDa (as indicated) using 5&nbsp;µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing&nbsp;conditions and using the 12-230 kDa separation system.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
CD117/c-kit Antibody [Unconjugated]
AF1356

Contact Information

Product PDFs

  Safety datasheet
  Manual - NBP3-14627
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human CD117/c-kit ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol KIT