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CD117/c-kit Antibody (OTI2B12) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI2B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

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View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71076AF350
Alexa Fluor 405
NBP2-71076AF405
Alexa Fluor 488
NBP2-71076AF488
Alexa Fluor 532
NBP2-71076AF532
Alexa Fluor 594
NBP2-71076AF594
Alexa Fluor 647
NBP2-71076AF647
Alexa Fluor 700
NBP2-71076AF700
Alexa Fluor 750
NBP2-71076AF750
Allophycocyanin
NBP2-71076APC
Biotin
NBP2-71076B
CoraFluor 1
NBP2-71076CL1
DyLight 350
NBP2-71076UV
DyLight 405
NBP2-71076V
DyLight 488
NBP2-71076G
DyLight 550
NBP2-71076R
DyLight 594
NBP2-71076DL594
DyLight 650
NBP2-71076C
DyLight 680
NBP2-71076FR
DyLight 755
NBP2-71076IR
FITC
NBP2-71076F
HRP
NBP2-71076H
Janelia Fluor 525
NBP2-71076JF525
Janelia Fluor 646
NBP2-71076JF646
Janelia Fluor 669
NBP2-71076JF669
PE
NBP2-71076PE
PerCP
NBP2-71076PCP
Unconjugated
NBP2-45508
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45508
Azide and BSA Free
NBP2-71076
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Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

CD117/c-kit Antibody (OTI2B12) [Janelia Fluor® 549] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 27-308 of human CD117/c-kit (NP_000213) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD117/c-kit Antibody (OTI2B12) [Janelia Fluor® 549]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KIT