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CD117/c-kit Antibody (ACK2) - BSA Free

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Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.
Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was ...read more
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b ...read more
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, GpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, IHC
Clone
ACK2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43359AF350
Alexa Fluor 405
NBP1-43359AF405
Alexa Fluor 488
NBP1-43359AF488
Alexa Fluor 532
NBP1-43359AF532
Alexa Fluor 594
NBP1-43359AF594
Alexa Fluor 647
NBP1-43359AF647
Alexa Fluor 700
NBP1-43359AF700
Alexa Fluor 750
NBP1-43359AF750
Allophycocyanin
NBP1-43359APC
Allophycocyanin/Cy7
NBP1-43359APCCY7
Biotin
NBP1-43359B
CoraFluor 1
NBP1-43359CL1
DyLight 350
NBP1-43359UV
DyLight 405
NBP1-43359V
DyLight 488
NBP1-43359G
DyLight 550
NBP1-43359R
DyLight 594
NBP1-43359DL594
DyLight 650
NBP1-43359C
DyLight 680
NBP1-43359FR
DyLight 755
NBP1-43359IR
FITC
NBP1-43359F
HRP
NBP1-43359H
Janelia Fluor 525
NBP1-43359JF525
Janelia Fluor 549
NBP1-43359JF549
Janelia Fluor 585
NBP1-43359JF585
Janelia Fluor 635
NBP1-43359JF635
Janelia Fluor 646
NBP1-43359JF646
Janelia Fluor 669
NBP1-43359JF669
PE
NBP1-43359PE
PE/Atto594
NBP1-43359PEATT594
PE/Cy5.5
NBP1-43359PECY55
PE/Cy7
NBP1-43359PECY7
PerCP
NBP1-43359PCP
mFluor Violet 450 SE
NBP1-43359MFV450
mFluor Violet 500 SE
NBP1-43359MFV500
mFluor Violet 610 SE
NBP1-43359MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-43359-0.05mg
BSA Free
NBP1-43359-0.025mg
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CD117/c-kit Antibody (ACK2) - BSA Free Summary

Immunogen
Mouse CD117
Marker
Hematopoietic Stem Cell Marker
Specificity
The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry < 0.125 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 26680736). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24161026).
Reviewed Applications
Read 1 Review rated 3
using
NBP1-43359 in the following applications:
Publications
Read Publications using
NBP1-43359 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (ACK2) - BSA Free

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD117/c-kit
Antibody (ACK2) - BSA
NBP1-43359
CD117/c-kit Antibody (ACK2) - BSA Free
Species: Hu, Mu, Gp
Applications: WB, Flow, ICC/IF, IHC, IP, IHC

Publications for CD117/c-kit Antibody (NBP1-43359)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, IHC-WhMt, WB.


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FLOW
(2)
ICC/IF
(2)
IF/IHC
(1)
IHC-P
(1)
IHC-WhMt
(1)
WB
(1)
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Human
(3)
Mouse
(3)
Rat
(1)
Guinea Pig
(1)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-43359 Applications Species
El-Naseery NI, Elewa YHA, El-Behery EI, Dessouky AA Human umbilical cord blood-derived mesenchymal stem cells restored hematopoiesis by improving radiation induced bone marrow niche remodeling in rats Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2023-07-15 [PMID: 37460043] (IHC-P, Rat)

Details:
1:1000 dilution 		IHC-P Rat
Takematsu E, Massidda M, Auster J et al. Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation Nature communications 2022-05-06 [PMID: 35523773] (ICC/IF, Mouse) ICC/IF Mouse
Schwerdtfeger LA, Tobet SA Sex differences in anatomic plasticity of gut neuronal-mast cell interactions Physiological reports 2021-10-01 [PMID: 34605201] (IF/IHC, Mouse) IF/IHC Mouse
Gude NA, Firouzi F, Broughton KM et al. Cardiac c-Kit Biology Revealed by Inducible Transgenesis. Circ. Res. 2018-04-10 [PMID: 29636378] (Human) Human
H Langhorst, R Jüttner, D Groneberg, A Mohtashamd, L Pelz, B Purfürst, KM Schmidt-Ot, A Friebe, FG Rathjen The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice Dis Model Mech, 2018-02-22;0(0):. 2018-02-22 [PMID: 29361518]
Schwerdtfeger LA, Ryan EP, Tobet SA. An Organotypic Slice Model For Ex Vivo Study of Neural, Immune and Microbial Interactions of Mouse Intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 2015-12-17 [PMID: 26680736] (IHC-WhMt, Mouse) IHC-WhMt Mouse
Zhang L, Tang J, Haines CJ et al. c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation. BMC Dev Biol. 2013-10-27 [PMID: 24161026] (ICC/IF, WB, Human) ICC/IF, WB Human
Meyer S, Maufort JP, Nie J et al. Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents PLoS One 2013-08-23 [PMID: 23967247] (FLOW, Human) FLOW Human
Fu W, Song B, Li W et al. Ultrastructural features and possible functional role of kit-positive interstitial cells in the guinea pig corpus cavernosum Int J Impot Res 2011-06-10 [PMID: 21654812] (FLOW, Guinea Pig) FLOW Guinea Pig

Review for CD117/c-kit Antibody (NBP1-43359) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-43359:
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ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD117/c-kit NBP1-43359
Enlarge 		3
reviewed by:
Verified Customer
 ICC Mouse 09/02/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedMouse Intestine (Ileum)
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD117/c-kit Antibody (NBP1-43359). (Showing 1 - 1 of 1 FAQs).

  1. Is there a blocking peptide that can be used as a control with the c-Kit antibody (ACK2) (NBP1-43359)?
    • NBP1-46164, NBP1-46249, P4713, P4834, H00003815-P01 are all c-kit proteins that could be suitable for blocking purposes.

Secondary Antibodies

 

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Blogs on CD117/c-kit.

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Immunocytochemistry CD117/c-kit NBP1-43359
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 Verified Customer
09/02/2015
Application: ICC
Species: Mouse
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Bioinformatics

Gene Symbol KIT
Entrez
Uniprot