![Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0007.jpg "Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EAFLNSQKAGKEKD corresponding to internal region according to NP_057671.2. |
| Specificity | No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein. |
| Predicted Species | Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CBX3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CBX3 Antibody (NB100-2418)Find related products by research area.
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| Gene Symbol | CBX3 |
| Entrez | |
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![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0009.jpg "Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NB100-2418-img0005.jpg "Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-Flow-Cytometry-NB100-2418-img0010.jpg "Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0006.jpg "Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0008.jpg "Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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