![Western Blot: Cbx2 Antibody (JE34-45) [NBP3-32114] - Western blot analysis of Cbx2 on different lysates with Rabbit anti-Cbx2 antibody (NBP3-32114) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: Mouse heart tissue lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32114_rabbit-cbx2-mab-je34-45-27820241524261.jpg "Western Blot: Cbx2 Antibody (JE34-45) [NBP3-32114] - Western blot analysis of Cbx2 on different lysates with Rabbit anti-Cbx2 antibody (NBP3-32114) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: Mouse heart tissue lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow |
| Clone | JE34-45 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide. (Uniprot: Q14781) |
| Localization | Nucleus, Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CBX2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cbx2 Antibody (NBP3-32114)Find related products by research area.
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| Gene Symbol | CBX2 |
![Flow Cytometry: Cbx2 Antibody (JE34-45) [NBP3-32114] - Flow cytometric analysis of HeLa cells labeling Cbx2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32114, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32114_rabbit-cbx2-mab-je34-45-29202416372149.jpg "Flow Cytometry: Cbx2 Antibody (JE34-45) [NBP3-32114] - Flow cytometric analysis of HeLa cells labeling Cbx2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32114, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")