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Caveolin-1 Antibody

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Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody [NBP3-23245] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled ...read more
Western Blot: Caveolin-1 Antibody [NBP3-23245] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caveolin-1 Antibody Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
The antibody was generated from a recombinant protein that includes amino acids in the N-terminal region of human Caveolin-1. [UniProt# Q03135]
Specificity
This antibody detects a 21 kDa protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAV1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Alternate Names for Caveolin-1 Antibody

  • CAV
  • CAV1
  • caveolae protein, 22kD
  • caveolin 1, caveolae protein, 22kDa
  • Caveolin1
  • Caveolin-1
  • cell growth-inhibiting protein 32
  • MSTP085
  • VIP21

Background

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CAV1