![Western Blot: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Western-Blot-NBP2-80617-img0001.jpg "Western Blot: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, ShSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | 7C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes |
| Localization | Cell Membrane |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CAV1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used. |
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| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Caveolin-1 Antibody (NBP2-80617)Find related products by research area.
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| Gene Symbol | CAV1 |
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80617-img0004.jpg "Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80617-img0007.jpg "Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80617-img0002.jpg "Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80617-img0003.jpg "Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80617-img0005.jpg "Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.")
![Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80617-img0006.jpg "Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin.")
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