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Cathepsin F Antibody [Unconjugated]

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Cathepsin F was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human Cathepsin F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2075) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cathepsin F was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human Cathepsin F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2075) at 5 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2075-SP
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Cathepsin F Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin F
Ala20-Asp484
Accession # Q9UBX1
Specificity
Detects human Cathepsin F in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cathepsin F is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSF
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2075 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin F Antibody [Unconjugated]

  • Cathepsin F
  • CATSFEC 3.4.22.41
  • CTSF
  • EC 3.4.22

Background

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin F Antibody (AF2075)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(2)
Western Blot
(1)
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(3)
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Bioinformatics

Gene Symbol CTSF
Uniprot