![Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0015.jpg "Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9. Amino Acid Sequence: ASTSPEDESPGSNPEPDATP |
| Specificity | Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD). NBP1-76961 can detect human and mouse isoform. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CASP9 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control |
|
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Publication using NBP1-76961 | Applications | Species |
|---|---|---|
| Marwarha G, Rosand o, Scrimgeour N et al. miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner Biomedicines 2021-12-25 [PMID: 35052722] (ELISA) | ELISA |
Secondary Antibodies |
Isotype Controls |
Research Areas for Caspase-9 Antibody (NBP1-76961)Find related products by research area.
|
|
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
|
The Ins and Outs of Survivin By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ... Read full blog post. |
|
Caspase-3, The Executioner of Apoptosis The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat... Read full blog post. |
|
Caspase 9 - an important apoptosis marker Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i... Read full blog post. |
|
A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0011.jpg "Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.")
![Western Blot: Caspase-9 Antibody [NBP1-76961] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, NBP1-76470 (1 ug/mL), Caspase 9, NBP1-76961 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0013.jpg "Western Blot: Caspase-9 Antibody [NBP1-76961] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, NBP1-76470 (1 ug/mL), Caspase 9, NBP1-76961 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0014.jpg "Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0006.jpg "Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0012.jpg "Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Simple Western BID Antibody [Unconjugated]](https://images.novusbio.com/images2/BID_AF860_Simple_Western_16992.jpg)
![Western Blot BID Antibody [Unconjugated]](https://images.novusbio.com/images2/BID_AF860_Western_Blot_22585.jpg)
![Western Blot BID Antibody [Unconjugated]](https://images.novusbio.com/images2/BID_AF860_Western_Blot_9663.jpg)
![Western Blot XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Western_Blot_5911.jpg)
![Simple Western XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Simple_Western_16640.jpg)
![Knockout Validated XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Knockout_Validated_22488.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")