Caspase-3 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa member of the peptidase C14A family of enzymes (1, 2, 3). It is widely expressed and is an integral component of the apoptotic cascade. Caspase-3 is considered to be the major executioner caspase; that is, the primary downstream mediator of apoptotic-associated proteolysis (2, 3, 4). Active Caspase-3 is known to utilize a Cys residue to cleave multiple substrates, including PARP, proIL-16, PKC-gamma & -δ, procaspases 6, 7 and 9, and beta -catenin (1). Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein (5, 6, 7). Normally, it is an inactive, cytosolic homodimer, but following an upstream signal that activates processing proteases, procaspase-3 undergoes proteolytic cleavage (1, 2, 8, 9). This generates an N-terminal 175 aa p20/20 kDa subunit plus a 102 aa C-terminal p12/12 kDa subunit, followed by further processing of the p20 subunit at Asp28 to generate a final p17 subunit (aa 29-175) (9). The p17 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p17/p12 heterodimer to form an active antiparallel homodimer. The p17 subunit contains the enzyme active site (aa 161-165), with an embedded catalytic Cys which is normally nitrosylated and inactive. Full activation requires both proteolytic processing and Cys163 denitrosylation (10). Multiple proteases can use Caspase-3 as a substrate including Caspase-6, -8, and -10, granzyme B, and Caspase-3 itself (9, 11, 12, 13). 

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila melanogaster.

We have publications tested in 4 applications: ICC, IHC, Proximity Extension Assay, Western Blot.

Showing Publications 1 - 10 of 22. Show All 22 Publications. Collapse Publications.

Publications using AF-605-NA	Applications	Species
Z Peng, B Gillissen, A Richter, T Sinnberg, MS Schlaak, J Eberle Enhanced Apoptosis and Loss of Cell Viability in Melanoma Cells by Combined Inhibition of ERK and Mcl-1 Is Related to Loss of Mitochondrial Membrane Potential, Caspase Activation and Upregulation of Proapoptotic Bcl-2 Proteins International Journal of Molecular Sciences, 2023-03-04;24(5):. 2023-03-04 [PMID: 36902392] (Western Blot, Human)	Western Blot	Human
S Kushalan, LC D'Souza, K Aloysius, A Sharma, S Hegde Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study International Journal of Environmental Research and Public Health, 2022-11-18;19(22):. 2022-11-18 [PMID: 36429955] (IHC, Drosophila melanogaster)	IHC	Drosophila melanogaster
Rova Virgana, Nur Atik, Julia Windi Gunadi, Evelyn Jonathan, Dona Erisa Ramadhani, Ray Sebastian Soetadji et al. MitoTEMPOL Inhibits ROS-Induced Retinal Vascularization Pattern by Modulating Autophagy and Apoptosis in Rat-Injected Streptozotocin Model Life (Basel) 2022-07-15 [PMID: 35888150]
Antonella Montinaro, Itziar Areso Zubiaur, Julia Saggau, Anna-Laura Kretz, Rute M. M. Ferreira, Omar Hassan et al. Potent pro-apoptotic combination therapy is highly effective in a broad range of cancers Cell Death & Differentiation 2022-03-01 [PMID: 34535764]
BA McKenzie, JP Fernandes, MAL Doan, LM Schmitt, WG Branton, C Power Activation of the executioner caspases-3 and -7 promotes microglial pyroptosis in models of multiple sclerosis J Neuroinflammation, 2020-08-29;17(1):253. 2020-08-29 [PMID: 32861242] (ICC, IHC, Western Blot, Human)	ICC, IHC, Western Blot	Human
YE Hadisaputr, N Cahyana, M Muchtaridi, R Lesmana, T Rusdiana, AY Chaerunisa, I Sufiawati, T Rostinawat, A Subarnas Apoptosis-mediated antiproliferation of A549 lung cancer cells mediated by Eugenia aquea leaf compound 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone and its molecular interaction with caspase receptor in molecular docking simulation Oncol Lett, 2020-03-19;19(5):3551-3557. 2020-03-19 [PMID: 32269629] (Western Blot, Human)	Western Blot	Human
MA Khan, T Shamma, S Kazmi, A Altuhami, HA Ahmed, AM Assiri, DC Broering Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants J Transl Med, 2020-03-31;18(1):147. 2020-03-31 [PMID: 32234039] (IHC, Mouse)	IHC	Mouse
H Kaihola, FG Yaldir, T Bohlin, R Samir, J Hreinsson, H Åkerud Levels of caspase-3 and histidine-rich glycoprotein in the embryo secretome as biomarkers of good-quality day-2 embryos and high-quality blastocysts PLoS ONE, 2019-12-19;14(12):e0226419. 2019-12-19 [PMID: 31856190] (Proximity Extension Assay, Human)	Proximity Extension Assay	Human
X Ma, F Cheng, K Yuan, K Jiang, T Zhu Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors Mol Med Rep, 2019-06-06;20(2):879-886. 2019-06-06 [PMID: 31173228] (Western Blot, Human)	Western Blot	Human
O'Brien, C E, Santos, P T Et al. Hypoxia-Ischemia and Hypothermia Independently and Interactively Affect Neuronal Pathology in Neonatal Piglets with Short-Term Recovery. Dev Neurosci 5/21/2019 [PMID: 31108487]
Zhenzhen Liu, Hongli Li, Lian He, Yu Xiang, Chengsen Tian, Can Li et al. Discovery of Small-Molecule Inhibitors of the HSP90-Calcineurin-NFAT Pathway against Glioblastoma Cell Chemical Biology 3/1/2019 [PMID: 30639261]
M Biernacki, W ?uczaj, I Jarocka-Ka, E Ambro?ewic, M Toczek, E Skrzydlews The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension Molecules, 2018-09-14;23(9):. 2018-09-14 [PMID: 30223427] (Western Blot, Rat)	Western Blot	Rat
Carl A Ascoli, Birte Aggeler Overlooked benefits of using polyclonal antibodies BioTechniques 2018-09-01 [PMID: 30089399]
Yuyi Wang, Chi Du, Nan Zhang, Mei Li, Yanyang Liu, Maoyuan Zhao et al. TGF‑ beta 1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosis Oncology Letters 2018-02-14 [PMID: 29552221]
AL DuMont, NP Cianciotto Stenotrophomonas maltophilia Serine Protease StmPr1 Induces Matrilysis, Anoikis, and Protease-Activated Receptor-2 Activation in Human Lung Epithelial Cells Infect. Immun., 2017-11-17;0(0):. 11/17/2017 [PMID: 28893914]
S Einsele-Sc, S Malmsheime, K Bertram, D Stehle, J Johänning, M Manz, PT Daniel, BF Gillissen, K Schulze-Os, F Essmann Bok is a genuine multi-BH-domain protein that triggers apoptosis in the absence of Bax and Bak and augments drug response J Cell Sci, 2016-04-13;0(0):. 2016-04-13 [PMID: 27076518] (Western Blot, Human)	Western Blot	Human
Luyet C, Schulze K, Sayar B, Howald D, Muller E, Galichet A Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PLoS ONE, 2015-03-06;10(3):e0119809. 3/6/2015 [PMID: 25748204]
Judith Knievel, Wolfgang A. Schulz, Annemarie Greife, Christiane Hader, Tobias Lübke, Ingo Schmitz et al. Multiple Mechanisms Mediate Resistance to Sorafenib in Urothelial Cancer International Journal of Molecular Sciences 2014-11-07 [PMID: 25387078]
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis, 2010-04-01;15(4):463-73. 2010-04-01 [PMID: 19997978] (Western Blot, Rat)	Western Blot	Rat
Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma. Mol. Cancer Ther., 2008-03-04;7(3):500-9. 2008-03-04 [PMID: 18319333] (Western Blot, Human)	Western Blot	Human
Engesaeter BØ, Bonsted A, Lillehammer T, Engebraaten O, Berg K, Maelandsmo GM Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines. Cancer Biol. Ther., 2006-11-19;5(11):1511-20. 2006-11-19 [PMID: 17012835] (Western Blot, Human)	Western Blot	Human
Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production. Proc. Natl. Acad. Sci. U.S.A., 2006-06-19;103(26):9982-7. 2006-06-19 [PMID: 16785446] (Western Blot, Human)	Western Blot	Human
Show All 22 Publications. Collapse Publications.

Array AF-605-NA

• Caspase-3 Antibodies
• Caspase-3 ELISA Kits
• Caspase-3 Kit
• Caspase-3 Lysates
• Caspase-3 Protein
• Caspase-3 ELISA
• Caspase-3 Proteins and Enzymes