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Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free

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Biological Strategies: Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by ...read more
Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were ...read more
Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation ...read more
Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse aCaspase-3 Antibody (31A1067) - (Pro and Active) (NB100-56708), for 1 hour at room temperature ...read more
Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were ...read more
An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Biological Strategies: Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane was probed with 0.1 ug/mL of mouse monoclonal Caspase-3 Antibody (31A1067) - ...read more
An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with ...read more
Western Blot: Mouse Monoclonal Caspase-3 Antibody (31A1067) - (Pro and Active) [IMGENEX: IMG-144A] [NB100-56708] - Whole cell lysates from MDA-MB-231, MCF-7, SUM149 and SUM159 cells were loaded with 50 ug/lane. 10% ...read more
Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - rOPN administration influenced the interaction & balance between Beclin 1 & Caspase‐3 at 24 h after ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - PEM induces YIPF2 upregulation & apoptosis in NSCLC cells.a, b H1792 (a) & H1299 (b) NSCLC cells were treated with PEM at various ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - PEM induces YIPF2 upregulation & apoptosis in NSCLC cells.a, b H1792 (a) & H1299 (b) NSCLC cells were treated with PEM at various ...read more
Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - Lipofection of top broccoletti-miR candidates does not influence basal & induced apoptosis.(A) BxPc-3 & ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - PEM induces apoptosis of NSCLC cells via YIPF2-TNFRSF10B axis.a, b A549 (a) & H1792 (b) cells were treated with PEM at 5.0 μM ...read more
Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - Cell numbers positively stained for NeuN, DCX, PCNA & caspase-3 in the subgranular region of the ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - NFATc1 regulates DcR3 expression at a transcriptional level. (A,B) Immunoblot analysis of whole-cell lysates & quantitative ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - rOPN administration elevated the expression of autophagy‐related proteins while suppressing apoptosis in rat brain at 24 h ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - RAB8 suppresses PEM-induced apoptosis of NSCLC cells by promoting the removing of TNFRSF10B from plasma membrane to cytoplasm.a, b ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - RAB8 suppresses PEM-induced apoptosis of NSCLC cells by promoting the removing of TNFRSF10B from plasma membrane to cytoplasm.a, b ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, ChHa, MaSpecies Glossary
Applications WB, Simple Western, Flow, HC, ICC/IF, IHC, MS, WB, CyTOF-ready, IHC, KD, KO
Clone
31A1067
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

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Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free Summary

Immunogen
This Caspase-3 Antibody (31A1067) - (Pro and Active) was developed against full-length recombinant human caspase-3 protein.
Specificity
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Electron Microscopy reported in scientific literature (PMID 27450722)
  • Flow Cytometry reported in scientific literature (PMID 27429862)
  • Hematoxylin and Eosin Stain reported in scientific literature (PMID 28186963)
  • Immunoblotting reported in scientific literature (PMID 28500555)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 23840553)
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 31771656)
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500. Use reported by customer review
  • Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (PMID 28500555)
  • Knockdown Validated reported in scientific literature (PMID 32867814)
  • Knockout Validated
  • Simple Western 1:50
  • Western Blot 1 - 5 ug/ml
Application Notes
The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caspase-3 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-56708 in the following applications:

Publications
Read Publications using
NB100-56708 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, Flow, HC, ICC/IF, IHC, MS, WB, CyTOF-ready, IHC, KD, KO

Publications for Caspase-3 Antibody (NB100-56708)(196)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Chinese Hamster, Mammal, Rabbit.

We have publications tested in 14 applications: Block/Neutralize, EM, FLOW, HEStain, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-P, KD, Simple Western, WB.


Filter By Application
Block/Neutralize
(2)
EM
(1)
FLOW
(4)
HEStain
(1)
IB
(1)
ICC/IF
(4)
IF/IHC
(2)
IHC
(1)
IHC-Fr
(4)
IHC-FrFl
(1)
IHC-P
(3)
KD
(1)
Simple Western
(1)
WB
(128)
All Applications
Filter By Species
Human
(91)
Mouse
(15)
Rat
(10)
Chicken
(3)
Chinese Hamster
(1)
Mammal
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 196. Show All 196 Publications.
Publications using NB100-56708 Applications Species
Sethy B, Upadhyay R, Narwanti I et al. Novel dual inhibitor targeting CDC25 and HDAC for treating triple-negative breast cancer. Apoptosis : an international journal on programmed cell death 2024-10-12 [PMID: 39395083]
Zhang Q, Zhang Q, Li H et al. Lithium Chloride Induces Apoptosis in Human Choroidal Melanoma Cells via the NOXA/Mcl-1 Axis and Induction of ER Stress Research Square Aug 28 2020 (WB, Human) WB Human
Han HJ, Sivaraman A, Kim M et al. HIF-1? inhibition by MO-2097, a novel chiral-free benzofuran targeting hnRNPA2B1 Journal of advanced research 2023-11-15 [PMID: 37977260] (WB, Human) WB Human
Kig C, Mertoglu E, Caliskan A et al. Selective and oxidative stress-mediated cell death of MCF-7 cell line induced by terpinolene Biologia 2021-06-12
Zhang Q, Zhang Q, Li H Lithium Chloride Induces Apoptosis in Human Choroidal Melanoma Cells via the NOXA/Mcl-1 Axis and Induction of ER Stress Research Square 2020-08-28 (WB, Human) WB Human
Büyükerkmen E, Atay E, Firat F et al. Effect of sugammadex administration on neural tube development in 48-h chick embryos Microscopy research and technique 2023-11-07 [PMID: 37933747] (ICC/IF, Chicken) ICC/IF Chicken
Rodriguez C Regulation of DNA end resection by cell stemness Thesis 2020-01-01 (WB) WB
Ning Mu, Yu Wang, Xiaopeng Li, Zhiyuan Du, Yingdi Wu, Min Su, Yingying Wang, Xiaoyang Sun, Ling Su, Xiangguo Liu Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells Cell Death & Disease 2023-09-30 [PMID: 37777549]
Tabanifar B, Moorthy A, Tsai HH et al. JNK mediates cell death by promoting the ubiquitination of the apurinic/apyrimidinic endonuclease APE1 Cell reports 2023-09-12 [PMID: 37703179] (WB, Human) WB Human
Show All 196 Publications.

Reviews for Caspase-3 Antibody (NB100-56708) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-56708:
Filter by Applications
WB
(2)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
WB 12/20/2023
View

Summary

ApplicationWestern Blot
LotG-8
  5
reviewed by:
Verified Customer
WB Human 01/24/2022
View

Summary

ApplicationWestern Blot
Sample TestedJurkat human acute T cell leukemia cell line
SpeciesHuman
Lot31A1067

Comments

CommentsPrimary antibody dilution 1:1000
  4
reviewed by:
Nayeli Paez-Martinez
IHC-Fr Mouse 04/23/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56708) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Caspase-3 Antibody (NB100-56708)

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Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Cleaved Caspase-3: A Marker of Programmed Cell Death
What are Caspases?Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environme...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
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Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
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D4-GDI (GDP dissociation inhibitor, RhoGD12)
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Showing 1-10 of 11 blog posts - Show all blog posts.
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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Yongkang Yang
12/20/2023
Application: WB
Species:

Verified Customer
01/24/2022
Application: WB
Species: Human

 
Nayeli Paez-Martinez
04/23/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CASP3