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Carbonic Anhydrase I/CA1 Antibody (0G10F8)

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Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB
Clone
0G10F8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Carbonic Anhydrase I/CA1 Antibody (0G10F8) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-261 of human Carbonic Anhydrase I/CA1 (NP_001729.1). KVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CA1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Carbonic Anhydrase I/CA1 Antibody (0G10F8)

  • CA1
  • CA-I
  • Car1
  • Carbonate dehydratase I
  • carbonic anhydrase 1
  • Carbonic anhydrase B
  • Carbonic Anhydrase I
  • carbonic anhydrase ICAB
  • carbonic dehydratase
  • EC 4.2.1.1

Background

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2. Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA1