I just read the data sheet of CNR1 lysate cat# H00001268-T01 and it says you can not determine the concentration. Can you tell me how much you need to run on a gel?

Name: Cannabinoid R1/CB1/CNR1 293T Cell Transient Overexpression Lysate
Brand: Novus Biologicals
SKU: H00001268-T01
Price: 429 USD

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This product is from our supplier Abnova and they recommends loading 15 ul per lane for their overexpression lysates.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB

Description	Quality control test: Transient overexpression cell lysate was tested with Anti-CNR1 antibody by Western Blots. Plasmid: pCMV-CNR1 full-length
Specificity	Cannabinoid R1/CB1/CNR1 293T Cell Transient Overexpression Lysate(Denatured)
Gene	CNR1

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Type	293T Cell Transient Overexpression
Protein State	Denatured

Cannabinoid R1/CB1/CNR1 293T Cell Transient Overexpression Lysate

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