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CaM Kinase II alpha Antibody

Images

 
Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Analysis of extracts from Hela using NB100-81830
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NB100-81830] - Staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using NB100-81830
Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Western blotting analysis using NB100-81830

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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CaM Kinase II alpha Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human CaM Kinase II alpha around amino acids 284~288 (Q-E-T-V-D).
Specificity
Detects endogenous levels of total CaMKII protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28912554).
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CaM Kinase II alpha Overexpression Lysate
Publications
Read Publications using
NB100-81830 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CaM Kinase II alpha Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaM Kinase II alpha Antibody (NB100-81830)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot