![Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0006.jpg "Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary. |
| Marker | Mesothelial Cell Marker |
| Predicted Species | Porcine (96%), Bovine (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CALB2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publications using NBP1-32244 | Applications | Species |
|---|---|---|
| Brokhman I, Xu J, Coles BLK et al. Dual embryonic origin of the mammalian enteric nervous system. Dev. Biol. 2018-11-22 [PMID: 30472119] (ICC/IF, Mouse) | ICC/IF | Mouse |
| Robinson CL, Chong ACN, Ashbrook AW et al. Male germ cells support long-term propagation of Zika virus Nat Commun 2018-05-29 [PMID: 29844387] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Calretinin Antibody (NBP1-32244)Find related products by research area.
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Using RPE65 as a tool to investigate ocular gene therapies While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life. Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALB2 |
![Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32244-img0010.jpg "Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen Section adult mouse hippocampus. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Frozen-NBP1-32244-img0017.jpg "Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen Section adult mouse hippocampus. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: Calretinin Antibody [NBP1-32244] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CALB2-transfected 293T cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0008.jpg "Western Blot: Calretinin Antibody [NBP1-32244] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CALB2-transfected 293T cells.")
![Western Blot: Calretinin Antibody [NBP1-32244] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0015.jpg "Western Blot: Calretinin Antibody [NBP1-32244] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Calretinin Antibody [NBP1-32244] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0005.jpg "Western Blot: Calretinin Antibody [NBP1-32244] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse fore brain. Calretinin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0007.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse fore brain. Calretinin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Frozen-NBP1-32244-img0013.jpg "Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse retina, using Calretinin antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0016.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse retina, using Calretinin antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")