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Calretinin Antibody (JM12-93)

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Western Blot: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of Calretinin on mouse brain tissue lysates with Rabbit anti-Calretinin antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Western Blot: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of Calretinin on mouse brain tissue lysates with Rabbit anti-Calretinin antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band ...read more
Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of paraffin-embedded rat brain tissue using anti-Calretinin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of paraffin-embedded human brain tissue using anti-Calretinin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of paraffin-embedded mouse brain tissue using anti-Calretinin antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
JM12-93
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Calretinin Antibody (JM12-93) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Calretinin aa 60-100. (SwissProt: P22676 Human; SwissProt: Q08331 Mouse; SwissProt: P47728 Rat)
Localization
Cuticular plate, cytosol, nucleus, synaptic membrane, dendrite, gap junction, neuron projection, parallel fiber to Purkinje cell synapse, stereocilium, synapse, terminal bouton.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CALB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Calretinin Antibody (JM12-93)

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calretinin
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NBP2-67206
Calretinin Antibody (JM12-93)
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Bioinformatics

Gene Symbol CALB2