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Calretinin Antibody (CALB2/2602)

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Western Blot: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Western Blot: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Formalin-fixed, paraffin-embedded human Cerebellum stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Protein Array: Calretinin Antibody (CALB2/2602) [NBP3-07365] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
CALB2/2602
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08323AF350
Alexa Fluor 405
NBP3-08323AF405
Alexa Fluor 488
NBP3-08323AF488
Alexa Fluor 532
NBP3-08323AF532
Alexa Fluor 594
NBP3-08323AF594
Alexa Fluor 647
NBP3-08323AF647
Alexa Fluor 700
NBP3-08323AF700
Alexa Fluor 750
NBP3-08323AF750
Allophycocyanin
NBP3-08323APC
Biotin
NBP3-08323B
CoraFluor 1
NBP3-08323CL1
DyLight 350
NBP3-08323UV
DyLight 405
NBP3-08323V
DyLight 488
NBP3-08323G
DyLight 550
NBP3-08323R
DyLight 594
NBP3-08323DL594
DyLight 650
NBP3-08323C
DyLight 680
NBP3-08323FR
DyLight 755
NBP3-08323IR
FITC
NBP3-08323F
HRP
NBP3-08323H
Janelia Fluor 525
NBP3-08323JF525
Janelia Fluor 549
NBP3-08323JF549
Janelia Fluor 585
NBP3-08323JF585
Janelia Fluor 635
NBP3-08323JF635
Janelia Fluor 646
NBP3-08323JF646
Janelia Fluor 669
NBP3-08323JF669
PE
NBP3-08323PE
PerCP
NBP3-08323PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-08323
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Calretinin Antibody (CALB2/2602) Summary

Description
200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08323)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human Calretinin protein fragment (around aa23-242) (exact sequence is proprietary) (Uniprot: P22676)
Localization
Cytoplasmic and Nuclear
Marker
Mesothelioma Marker
Specificity
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CALB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Calretinin Antibody (CALB2/2602)

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calretinin
Antibody (CALB2/2602)
NBP3-07365
Calretinin Antibody (CALB2/2602)
Species: Hu
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Bioinformatics

Gene Symbol CALB2
Uniprot